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【目的】小麦品种Saar由CIMMYT育成,在欧洲、亚洲和南美洲对小麦叶锈、条锈和白粉病均表现出很高的成株抗性,发掘其成株抗叶锈QTL对于选育持久抗锈品种有重要作用。【方法】以Avocet与Saar杂交的109个F6代重组自交系为材料,利用142个SSR标记和209DArT(Diversity Arrays Technology)标记构建连锁图,对Saar和Avocet的成株抗性进行QTL分析。试验材料于2006-2007年度种植在河北保定和河南新乡两个试验点,调查各个家系对叶锈病的成株抗性。【结果】由351个位点组成的遗传连锁图,覆盖小麦21个连锁群,全长3083cM。采用复合区间作图法进行叶锈成株抗性的QTL分析,在1BL、2DS、5BL、6AL和7DS染色体上发现了5个抗叶锈病QTL,分别解释4.5%～6.4%、12.2%～12.5%、4.9%～11.2%、4.9%～7.8%和14.0%～67.6%的表型变异。【结论】叶锈成株抗性基因及其紧密连锁分子标记的发掘,将为小麦抗叶锈病育种的分子标记辅助选择(MAS)提供理论和技术支持。
【Objective】 The wheat variety Saar was bred by CIMMYT and showed high resistance to plant leaf rust, stripe rust and powdery mildew in Europe, Asia and South America. Rust-resistant varieties have an important role. 【Method】 The 109 F6 generation inbred lines crossed with Avocet and Saar were used as materials to construct QTLs for linkage analysis between Saar and Avocet using 142 SSR markers and 209 DArT (Diversity Arrays Technology) markers. The test materials were planted in two test sites in Baoding, Hebei and Xinxiang, Henan in 2006-2007 to investigate the adult plant resistance to leaf rust. 【Result】 The genetic linkage map consisting of 351 loci covered 21 linkage groups of wheat with a total length of 3083cM. Five QTLs for leaf rust resistance were found on the chromosomes 1BL, 2DS, 5BL, 6AL and 7DS using the composite interval mapping method, which explained 4.5% -6.4% and 12.2% ~ 12.5 respectively %, 4.9% ~ 11.2%, 4.9% ~ 7.8% and 14.0% ~ 67.6% of the phenotypic variation. 【Conclusion】 Discovery of the leaf rust-resistant genes and their close-linkage molecular markers will provide theoretical and technical support for the molecular marker-assisted selection (MAS) of wheat leaf rust resistance breeding.