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以菠萝[Ananascomosus(L.)Merr.]吸芽上的叶基部组织为材料,研究了2,4-D、NAA、BA、TDZ在菠萝愈伤组织诱导体细胞胚过程中的影响。结果表明,2,4-D在体细胞胚诱导过程中起着极为重要的作用,其适宜浓度为5mg/L,适当浓度(1mg/L)的BA在降低畸形胚比例和增加体细胞胚发生率方面有重要作用,但BA也会促进愈伤组织分化不定芽;低浓度的TDZ与BA效果相似,在不同生长调节物质处理组合中,MS+0.01mg/LTDZ+5mg/L2,4-D培养基的体细胞胚发生率最高(97%)。低浓度NAA(2￣5mg/L)利于非胚性愈伤组织的增殖,而高浓度的NAA虽能促进体细胞胚发生,但这种体细胞胚很难继续增殖,在萌发时只生根不发芽。成熟体细胞胚在MS+0.2%活性炭上30d时的转株率为22.7%。
The effects of 2,4-D, NAA, BA and TDZ on somatic embryogenesis induced by pineapple callus were studied using leaf basal tissue on the shoot of Ananascomosus (L.) Merr. The results showed that 2,4-D played an important role in the somatic embryogenesis induction. The optimum concentration of 2,4-D was 5mg / L. BA at a proper concentration (1mg / L) reduced the proportion of abnormal embryos and increased the occurrence of somatic embryos But BA also promoted adventitious bud differentiation of callus. TDZ with low concentration had the similar effect with BA. MS + 0.01mg / LTDZ + 5mg / L2,4-D The highest incidence of somatic embryos in culture medium (97%). Low concentration of NAA (2 ~ 5mg / L) is conducive to the proliferation of non-embryogenic callus, while high concentrations of NAA can promote somatic embryogenesis, but this is difficult to continue proliferation of somatic embryos, only rooting in the germination germination. Mature somatic embryos on MS + 0.2% activated charcoal on the 30d when the strain rate was 22.7%.