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了解肿瘤坏死因子α（ＴＮＦ-α）在人体疾病发生中的作用．方法：建立了ＴＮＦ-α的双抗体放射免疫分析法（ＲＩＡ）．结果：方法的批内及批间ＣＶ分别为５．７６％±１．７３％和６．０８％±１．９１％．灵敏度为５．４ｐｍｏｌ／Ｌ．临床应用结果显示，癌症、细菌感染及脑血管病病人的血清ＴＮＦ-α值显著高于正常人（Ｐ＜０．０１），败血症、脑缺氧症新生儿的血清ＴＮＦ-α值显著高于正常新生儿（Ｐ＜０．０１）．结论：测定血清ＴＮＦ-α值有助于临床疾病的诊断及治疗．
To understand the role of tumor necrosis factor alpha (TNF-alpha) in the development of human diseases. Methods: A double antibody radioimmunoassay (RIA) was established for TNF-α. Results: The intra-assay and inter-assay CV were 5.76% ± 1.73% and 6.08% ± 1.91%, respectively. Sensitivity of 5.4 pmol / L. The results of clinical application showed that serum TNF-α in patients with cancer, bacterial infection and cerebrovascular disease was significantly higher than that in normal people (P <0.01). Serum TNF-α in neonates with sepsis and cerebral hypoxia was significantly higher Normal newborns (P <0.01). Conclusion: The determination of serum TNF-α is helpful for the diagnosis and treatment of clinical diseases.