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目的:探讨CD4+CD25+调节性T细胞在糖尿病发病中的作用及机制。方法:选取BALB/c小鼠,每日腹腔注射链脲佐菌素(STZ)40mg/kg,连续5天,建立1型糖尿病模型;从小鼠内眦静脉采血,检测血糖;取小鼠尿液,检测尿糖;流式细胞术检测两种小鼠感染不同时间脾细胞悬液中CD4+T细胞和CD4+CD25+T细胞百分含量;ELISA法检测脾细胞培养上清IFN-γ和IL-10水平。结果:BALB/c小鼠的IFN-γ水平在注射STZ第2周和4周明显增高(P<0.05);IL-10水平在注射STZ第2周和4周明显降低(P<0.05);CD4+T细胞数量在注射STZ第2周未见明显升高,第4周水平明显升高(P<0.05);CD4+CD25+T细胞数量在注射STZ第2周和4周明显降低(P<0.05)。结论:CD4+CD25+调节性T细胞通过抑制Th1型细胞因子分泌,分泌IL-10等细胞因子,防止糖尿病的发生,维持自身耐受。
Objective: To investigate the role and mechanism of CD4 + CD25 + regulatory T cells in the pathogenesis of diabetes mellitus. Methods: BALB / c mice were injected intraperitoneally with streptozotocin (STZ) 40mg / kg for 5 consecutive days to establish type 1 diabetes mellitus model. Blood was collected from the internal canthal vein of mice to detect blood glucose. Urine , Urine glucose was detected. The percentages of CD4 + T cells and CD4 + CD25 + T cells in spleen cell suspension were detected by flow cytometry at different time points. The levels of IFN-γ and IL- -10 level. Results: The levels of IFN-γ in BALB / c mice were significantly increased at 2 weeks and 4 weeks after injection of STZ (P <0.05). The levels of IL-10 in 2 weeks and 4 weeks after STZ injection were significantly decreased (P <0.05) The number of CD4 + T cells in the second week and the fourth week after injection of STZ were not significantly increased (P <0.05) <0.05). Conclusion: CD4 + CD25 + regulatory T cells can prevent the occurrence of diabetes and maintain their tolerance by inhibiting the secretion of Th1-type cytokines and IL-10 and other cytokines.