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稻曲病近年来逐步上升为世界多个水稻产区的主要病害之一。利用丰富的抗性种质资源进行抗性品种选育是控制稻曲病害的重要手段。通过在四川省进行三年病圃筛选试验,我们从843份水稻材料中鉴定出179份完全不感稻曲病的抗性材料和两份高感材料(病穗率高达50%以上),其余材料的病穗率介于0.1%~48.8%之间,55.1%的病穗只有一个稻曲球,而高感稻曲病的蒲江6号单穗稻曲球高达38粒。然后我们选取36份材料进行多点多期播种,进一步验证其稻曲病抗感性。结果表明,有3份材料在所有试验中都不感稻曲病,18份材料偶发稻曲病呈现高抗,蒲江6号仍然表现为高感。我们用450对SSR引物对其中35份材料与蒲江6号进行多态性分析,发现其中12份材料与蒲江6号的多态性达10%以上,其中最高为ITAT144(16.1%),其次是泸香90-2(14.8%)和Domsia-2(14.4%)。因此,我们选择这些材料与蒲江6号杂交构建基因定位与克隆群体。
False false smut in recent years gradually increased to more than one of the world’s major rice-producing areas of the disease. Breeding resistant varieties using abundant resistant germplasm resources is an important method to control the disease of rice false smut. Through three-year disease screening test in Sichuan Province, we identified 179 non-rice false smut-resistant materials and two high-sensitive materials (disease panicle rate as high as 50%) out of 843 rice materials. The remaining materials The incidence of panicle disease ranged from 0.1% to 48.8%, and 55.1% of panicle disease had only one rice curl ball, while 38 of Pujiang No.6 single panicle rice ball highly susceptible to rice germplasm disease was 38. Then, we selected 36 materials for multi-point and multi-period sowing to further verify their false smut resistance. The results showed that 3 materials did not show false smut in all the experiments, 18 were highly resistant to the incidental rice false smut, and Pujiang 6 still showed high susceptibility. We analyzed the polymorphism of 35 materials and Pujiang 6 with 450 pairs of SSR primers and found that 12 of them were polymorphic with Pujiang 6 with the highest percentage being ITAT144 (16.1%), followed by Lu Hong 90-2 (14.8%) and Domsia-2 (14.4%). Therefore, we selected these materials to hybridize with Pujiang 6 to construct a gene mapping and clonal population.