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目的探讨９９Ｔｃｍ甲氧基异丁基异腈（ＭＩＢＩ）作为再灌注损伤示踪剂的可能性。方法７５只ＳＤ大鼠和６只新西兰兔分假手术对照和再灌注损伤（ＲＰＩ）２组。ＲＰＩ组结扎主动脉和下腔静脉，致双下肢缺血１５ｈ。测量再灌注过程中心率、动脉压、血气含量和脏器组织９９ＴｃｍＭＩＢＩ和２０１Ｔｌ摄取和残留。分析ＯＨ－自由基清除剂、ＯＨ－生成系统和阴离子化合物对脏器组织摄取９９ＴｃｍＭＩＢＩ的影响。结果ＲＰＩ和假手术组的心率、血压和血气含量无明显差别。再灌注时，肝、肺、心、肾和下肢缺血后骨骼肌早期摄取９９ＴｃｍＭＩＢＩ呈两次增加（再灌注后第１和第３０ｍｉｎ）。与对照组比较，再灌注３０ｍｉｎ心、肺、肾和下肢缺血后骨骼肌９９ＴｃｍＭＩＢＩ摄取分别平均增加了１４１％、８５％、８６％和１７５％；相反，相应脏器组织２０１Ｔｌ摄取平均减少了６６％、３１％、４４％和８％。正常骨骼肌摄取９９ＴｃｍＭＩＢＩ减少１９％，而２０１Ｔｌ摄取增加３３％。氧自由基和抗氧自由基仅能部分影响脏器组织摄取９９ＴｃｍＭＩＢＩ，而阴离子化合物可明显影响９９ＴｃｍＭＩＢＩ摄取。ＲＰＩ组织残留９９ＴｃｍＭＩＢＩ比对照组减低。ＲＰＩ的９９ＴｃｍＭＩＢ?
Objective To investigate the possibility of 99Tcm-methoxyisobutylisonitrile (MIBI) as a tracer for reperfusion injury. Methods 75 SD rats and 6 New Zealand rabbits were divided into sham operation control and reperfusion injury (RPI) groups. RPI group ligation of the aorta and inferior vena cava, resulting in lower limb ischemia 1 5h. Measurement of heart rate during reperfusion, arterial pressure, blood gas and tissue 99TcmMIBI and 201Tl intake and residual. The effects of OH-radical scavenger, OH-generating system and anionic compounds on 99Tcm -MIBI uptake in visceral tissues were analyzed. Results There was no significant difference in heart rate, blood pressure and blood gas between RPI group and sham operation group. Reperfusion, liver, lung, heart, kidney and lower limb ischemia after skeletal muscle uptake 99TcmMIBI increased twice (1 and 30 min after reperfusion). Compared with the control group, the uptake of 99TcmMIBI in skeletal muscles increased by 141%, 85%, 86% and 175% respectively after 30 min of reperfusion, but on the contrary, the average intakes of 201Tl in corresponding organs decreased 66%, 31%, 44% and 8%. Normal skeletal muscle uptake of 99TcmMII reduced 19%, while 201Tl uptake increased 33%. Oxygen free radicals and antioxidant free radicals can only affect part of the organ tissue uptake of 99TcmMII, and anionic compounds can significantly affect 99TcmMIBI uptake. RPI tissue residue 99TcmMIBI reduced compared with the control group. RPI 99TcmMIB?