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目的 了解肝硬化患者血清一氧化氮（ＮＯ） 与肿瘤坏死因子－ α（ＴＮＦ－ α） 的变化，并探讨二者之间的关系。方法 用Ｇｒｉｅｓｓ 方法及放免法分别检测５０ 例肝硬化及３０ 例正常人血清ＮＯ 与ＴＮＦ－ α水平。结果 肝硬化患者与正常人比较，其血清ＮＯ 水平升高（ Ｐ＜ ０ ．００１） ，有腹水患者较无腹水者为高（ Ｐ＜ ０ ．０５） ，且ＮＯ 水平随Ｃｈｉｌｄ － Ｐｕｇｈ Ａ、Ｂ、Ｃ 分级的次序而增加。同时肝硬化患者血ＴＮＦ－ α亦明显增加（ Ｐ＜ ０ ．００１） ，并与ＮＯ 呈正相关（ｒ ＝ ｏ ．６２ ，Ｐ＜ ０ ．００１） 。结论 肝硬化患者血ＮＯ 升高，有腹水者更高，且ＮＯ 水平随Ｃｈｉｌｄ － Ｐｕｇｈ Ａ、Ｂ、Ｃ 分级的次序而增加，提示ＮＯ 可能参与了肝硬化高动力循环与腹水的形成，并能反映患者的预后。同时ＴＮＦ－ α亦升高，且与ＮＯ 呈正相关，为ＴＮＦ－ α诱导ＮＯ 生成提供了依据
Objective To investigate the changes of serum nitric oxide (NO) and tumor necrosis factor - α (TNF - α) in cirrhotic patients and explore the relationship between the two. Methods Serum levels of NO and TNF-α in 50 cirrhotic patients and 30 normal controls were detected by Griess method and radioimmunoassay. Results Compared with normal controls, serum levels of NO in patients with cirrhosis increased (P <0.001), those with ascites were significantly higher than those without ascites (P <0.05), and the level of NO was positively correlated with Child - Pugh A, B , C classification order and increase. At the same time, serum TNF-α in patients with cirrhosis was also significantly increased (P <0.001), and positively correlated with NO (r = 0.62, P <0.001). Conclusions Patients with liver cirrhosis have elevated blood levels of NO, higher levels of ascites and increased levels of NO with the order of Child - Pugh A, B, C grading, suggesting that NO may be involved in the formation of hyperdynamic circulation and ascites in cirrhotic patients. Reflects the patient’s prognosis. At the same time, TNF-α also increased, and it was positively correlated with NO, which provided a basis for NO production induced by TNF-α