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目的对SD大鼠胚胎后肾间充质细胞(MMSCs)进行分离培养,并检测其生物学特性。方法采用胚胎后肾微组织块接种培养法,检测其生长曲线和生长周期,并进行细胞表面抗原和成骨能力的鉴定。结果MMSCs体外培养生长状况良好,具有活跃的增殖能力。细胞周期显示90%以上细胞处于G0/G1期。表面抗原检测绝大多数细胞阳性表达波形蛋白(vimen-tin)和纤维连接蛋白(fibronectin),几乎不表达CD34。在体外能够向成骨细胞分化。结论原代分离、培养的细胞为MMSCs,细胞纯度高、扩增迅速、生物学性状稳定,可为组织工程提供比较理想的种子细胞。
OBJECTIVE: To isolated and culture the SD rat embryonic kidney-derived mesenchymal cells (MMSCs) and test their biological characteristics. Methods The embryonic kidney tissue culture inoculation method was used to detect the growth curve and growth cycle. Cell surface antigen and osteogenic capacity were identified. Results MMSCs grew well in vitro with active proliferative capacity. Cell cycle shows that more than 90% of cells are in G0 / G1 phase. SURFACE ANTIBODY DETECTION Most cells express vimen-tin and fibronectin, rarely expressing CD34. Differentiate into osteoblasts in vitro. Conclusion Primary isolated and cultured cells are MMSCs with high purity, rapid amplification and stable biological characters, which can provide ideal seed cells for tissue engineering.