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目的 探讨红藻氨酸 (KA)诱导性癫痫发作中相关脑区白细胞介素 6(IL 6)表达的时空规律和细胞学特征及其与c Fos的比较。方法 采用惊厥剂量KA(1 0mg·kg- 1 ,ip)诱导大鼠癫痫发作 ,记录行为 ,并分别取KA处理后 1 5、30min ,1、3、6、1 2及 2 4h的大鼠脑片 ,用免疫组化方法观察海马结构、梨状区及大脑皮层等相关脑区IL 6免疫反应 (IL 6ir)的变化特征 ,并与相应时空内的c Fos免疫反应 (Fos ir)相比较。结果 IL 6和c Fos均出现了在癫痫发作早期的快速表达 ,但IL 6ir的早期反应更为明显。KA后 1 5min海马结构内若干血管壁出现了明确的IL 6ir,30min时海马各亚区、梨状区、大脑皮层部位开始出现较强的IL 6ir阳性神经元 ,其数量明显多于阳性胶质细胞 ,KA后 3h以上各脑区IL 6ir进一步增强 ,6h后阳性神经元免疫反应性减弱 ,1 2～2 4h继续减弱 ,但阳性胶质细胞数量及IL 6ir强度变化不明显。与IL 6ir相比 ,Fos ir的变化较为短暂并且强度较弱 ,KA后 30min ,齿状回颗粒细胞层、海马各亚区锥体细胞层及梨状区等部位出现了微弱的Fos ir。结论 KA诱导癫痫发作过程中IL 6在相关脑区神经元内的表达迅速增加 ,明显早于c Fos,表达水平与癫痫的严重程度成正相关
OBJECTIVE: To investigate the spatiotemporal and cytological characteristics of interleukin-6 (IL-6) in the brain regions associated with kainic acid (KA) -induced seizures and their comparison with c Fos. Methods The seizure and recording behavior of rats were induced by seizure dose of KA (10 mg · kg-1, ip) and the brain of rats at 1, 5, 30min, 1, 3, 6, 12 and 24 hours after KA treatment The changes of IL 6 immunoreactivity in hippocampal formation, piriform area and cerebral cortex were observed by immunohistochemistry and compared with that of Fos ir in corresponding spatiotemporal space. Results Both IL 6 and c Fos showed rapid expression in the early stage of seizure, but the early response of IL 6ir was more obvious. At 15 min after KA, certain IL 6ir appeared in several blood vessel walls in hippocampal formation. At 30 min, IL 6 ir positive neurons began to appear in sub-regional, piriform and cortical regions of hippocampus, which was significantly more than that of positive glial After 6h, the IL 6ir in each brain region was further enhanced. After 6h, the immunoreactivity of the positive neurons was weakened and continued to weaken after 1-24 hours. However, there was no obvious change in the number of glial cells and the intensity of IL 6ir. Compared with IL 6ir, Fos ir changes more transiently and has weaker intensity. After 30min KA, Fos ir appears weakly in the granular layer of dentate gyrus, the pyramidal cell layer of hippocampus and the pyramidal area of hippocampus. Conclusions The expression of IL-6 in neurons of relevant brain regions in KA-induced seizures increased rapidly, significantly earlier than that of c Fos, and the expression level was positively correlated with the severity of epilepsy