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利用细胞内的钙离子成像技术,研究了多巴胺对鲫鱼视网膜H1型水平细胞上L型电压门控钙离子通道的调控特性。研究发现,在不同胞外pH(6.8、7.4、8.0)条件下,不同浓度多巴胺(50和500μmol/L、1 mmol/L)对L型电压门控钙离子通道均具有上调作用。50μmol/L多巴胺在不同pH下的上调程度没有显著性差异;高浓度多巴胺(500μmol/L和1 mmol/L)在pH8.0条件下比pH6.8和7.4条件下具有更显著的上调作用。这些结果表明,在鲫鱼视网膜H1型水平细胞上,多巴胺对电压门控钙离子通道的调控与胞外pH环境密切相关。在光照情况下,L型电压门控钙离子通道活性被相对高浓度多巴胺上调,并被胞外碱性环境协同增强。这种协同增强效应有助于阐明视网膜对光反应的信息传递机制。
The effects of dopamine on the regulation of L-type voltage-gated calcium channels on the level of retinal H1-type cells in Carassius auratus were studied by intracellular calcium imaging. It was found that dopamine (50 and 500 μmol / L, 1 mmol / L) had an up-regulation of L-type voltage-gated calcium channel under different extracellular pH (6.8, 7.4 and 8.0) There was no significant difference in up-regulation of 50μmol / L dopamine at different pH values. High concentrations of dopamine (500μmol / L and 1 mmol / L) had more significant up-regulation under pH8.0 than pH6.8 and 7.4. These results indicate that the regulation of voltage-gated calcium channels by dopamine is closely related to the extracellular pH environment on crucian-type H1 retinal cells. In light conditions, L-type voltage-gated calcium channel activity is upregulated by relatively high concentrations of dopamine and is synergistically enhanced by the extracellular alkaline environment. This synergistic effect helps to elucidate the mechanism of information transmission through the retina to photoreactions.