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在２６～３４℃范围内甘薯茎尖分生组织离体培养均能成苗，在此温度范围两端，因品种不同成苗率有差别。最适温度为２９±１℃。２２～２４℃下甘薯茎尖分生组织虽能生长，但不能成苗。培养基ｐＨ４．６～７．０对较易培养成苗的甘薯品种成苗率无明显影响，高ｐＨ值对较难培养成苗的品种有影响。培养基琼脂浓度０．４～０．７％均适于甘薯茎尖分生组织培养。
In the range of 26 ~ 34 ℃ sweet potato shoot apical meristem tissue can be cultured in vitro, at both ends of the temperature range, due to different varieties have different seedling rates. The optimum temperature is 29 ± 1 ℃. 22 ~ 24 ℃ sweetpotato shoot apical meristematic although it can grow, but not seedlings. Medium pH4.6-7.0 had no significant effect on the seedling rate of sweet potato varieties which were easier to grow into seedlings, and high pH value had an impact on the varieties which were more difficult to grow. Medium agar concentration 0.4 ~ 0.7% are suitable for sweet potato shoot tip meristem culture.