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目的 研究HBVC基因启动子 (BasicCorePromoter,BCP)基因变异与前S1(Pre -S1)抗原的关系以及BCP基因变异与HBVDNA含量的关系。方法 用PCR -微板核酸杂交ELISA技术检测 94例HBeAg阳性乙肝患者的BCP中nt 176 2A -T和nt 176 4G -A的基因突变 ,酶联免疫吸附分析 (ELISA)检测Pre -S1抗原 ,PCR荧光定量技术检测HBVDNA。结果 在 94例HBeAg阳性乙肝患者的血清中 ,Pre-S1抗原阳性有 6 5例 ,其中BCP阳性 5 1例 ,BCP变异率为 78.5 % ;Pre -S1抗原阴性有 2 9例 ,其中BCP阳性 2 3例 ,BCP变异率为 79.3%。BCP基因突变血清中HBVDNA拷贝数明显高于非BCP基因突变血清中HBV含量。结论 在e抗原阳性乙肝患者中 ,Pre -S1抗原的存在与BCP的变异无相关性 ,但BCP基因变异与HBVDNA拷贝数明显相关 ,可反映乙肝病情的严重程度及其愈后、转归。
Objective To study the relationship between the mutation of HBV C gene promoter (BCP) and Pre S1 antigen and the relationship between BCP gene mutation and HBVDNA content. Methods The gene mutations of nt 176 2A-T and nt 176 4G-A in BCP of 94 patients with HBeAg-positive hepatitis B were detected by PCR-microplate nucleic acid hybridization ELISA. Pre-S1 antigen was detected by enzyme-linked immunosorbent assay (ELISA) Fluorescence quantitative detection of HBVDNA. Results Among the 94 patients with HBeAg-positive hepatitis B, the Pre-S1 antigen was found to be positive in 65 cases, including 51 cases of BCP positive and 78.5% of BCP. The Pre-S1 antigen negative was found in 29 cases, of which BCP was positive 3 cases, BCP mutation rate was 79.3%. The HBV DNA copy number of BCP gene mutation serum was significantly higher than that of non-BCP gene mutation serum. Conclusion There is no correlation between the Pre-S1 antigen and BCP in e antigen-positive hepatitis B patients. However, the BCP gene mutation is significantly correlated with the copy number of HBVDNA, which can reflect the severity of hepatitis B and its prognosis.