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本文运用RAPD对辽宁、山东、江苏、福建、广东、广西等7个地理群体的文蛤进行了分析。从40条随机引物中,筛选出11个扩增效果好、条带清晰的随机引物进行扩增。结果显示,7群体内遗传多样性丰富,遗传相似度在0.7251~0.8972之间,依次排列为:辽宁营口(0.8972)>广东湛江(0.8756)>山东日照(0.8729)>山东潍坊(0.8643)>福建云霄(0.8245)>江苏启东(0.7653)>广西合浦(0.7251)。群体间遗传距离指数及用UPGMA和NJ法构建的分子系统树结果表明,山东日照与山东潍坊两群体的亲缘关系较近,广东湛江群体与其它群体亲缘关系相对较远。
In this paper, RAPD was used to analyze the clam of seven geographical groups in Liaoning, Shandong, Jiangsu, Fujian, Guangdong and Guangxi. From 40 random primers, 11 random primers with good amplification effect and clear bands were screened for amplification. The results showed that the genetic diversity of 7 populations was rich and the genetic similarity was between 0.7251 and 0.8972, ranked as follows: Liaoning Yingkou (0.8972)> Guangdong Zhanjiang (0.8756)> Shandong Rizhao (0.8729)> Shandong Weifang (0.8643)> Fujian Clouds (0.8245)> Jiangsu Qidong (0.7653)> Guangxi Hepu (0.7251). The genetic distance index and the molecular phylogenetic tree constructed by UPGMA and NJ methods showed that the genetic relationship between Shandong Rizhao and Shandong Weifang was close, and the Zhanjiang population in Guangdong was relatively distant from other groups.