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目的探讨牛磺酸对糖尿病(diabetes mellitus,DM)大鼠视网膜Müller细胞的影响及机制。方法取雄性SD大鼠随机分为对照组、DM组、牛磺酸组、PBS组(n=6/组),对照组正常大鼠腹腔注射柠檬酸缓冲液,DM组大鼠腹腔注射链脲佐菌素诱导的DM模型,牛磺酸组DM大鼠腹腔注射牛磺酸,PBS组DM大鼠腹腔注射PBS。3个月后试剂盒检测视网膜还原型谷胱甘肽(reduced glutathione hor-mone,GSH)及含量丙二醛(malondialdehyde,MDA),Western blot检测视网膜胶质纤维酸性蛋白(glial fi-brillary acidic protein,GFAP)及谷氨酰胺合成酶(glutamine synthetase,GS)表达。结果对照组MDA及GSH含量分别为(3.52±0.51)nmol/mg prot及(95.47±8.73)mg/g prot;DM组及PBS组MDA含量分别为(6.72±0.75)、(7.22±0.54)nmol/mg prot,较对照组明显增高(P<0.05),而GSH含量分别为(46.38±5.31)、(63.21±6.48)mg/g prot,较对照组明显降低(P<0.05);牛磺酸组MDA及GSH含量分别为(4.01±0.38)nmol/mg prot、(85.39±8.47)mg/g prot较对照组,差异均无统计学意义(P>0.05)。与对照组相比,DM及PBS组视网膜GFAP表达上调,GS表达下调,牛磺酸组GFAP及GS表达无明显变化。结论抑制DM视网膜氧化应激是牛磺酸保护Müller细胞,上调GS表达的重要机制之一。
Objective To investigate the effect and mechanism of taurine on retinal Müller cells in diabetic mellitus (DM) rats. Methods Male Sprague-Dawley rats were randomly divided into control group, DM group, taurine group and PBS group (n = 6 / group). The control rats were injected intraperitoneally with citrate buffer solution, In the DM model induced by ZO, taurine was injected intraperitoneally into DM rats in taurine group. PBS was injected intraperitoneally into DM rats in PBS group. After 3 months, the reduced glutathione hormones (GSH) and malondialdehyde (MDA) were detected by the kit and the expressions of glial fi-brillary acidic protein , GFAP) and glutamine synthetase (GS). Results The contents of MDA and GSH in the control group were (3.52 ± 0.51) nmol / mg prot and (95.47 ± 8.73) mg / g prot, respectively. The MDA contents in the DM group and the PBS group were 6.72 ± 0.75 and 7.22 ± 0.54 nmol / mg prot, which was significantly higher than that of the control group (P <0.05), while the content of GSH was (46.38 ± 5.31) and (63.21 ± 6.48) mg / The levels of MDA and GSH were (4.01 ± 0.38) nmol / mg prot and (85.39 ± 8.47) mg / g prot were lower than those of the control group (P> 0.05). Compared with the control group, the GFAP expression in the retina of DM and PBS groups was up-regulated and the expression of GS was down-regulated. There was no significant change in the expression of GFAP and GS in taurine group. Conclusion Inhibition of DM retinal oxidative stress is one of the important mechanisms by which taurine protects Müller cells and up-regulates GS expression.