论文部分内容阅读
目的构建变形链球菌UAl59密度感应相关的comD基因缺陷菌株,为进一步研究该基因功能做准备。方法根据同源重组原理,利用变形链球菌UAl59comD基因同源重组DNA片段,采用电击转化方法获取转化菌落,通过形态学观察、生化特性检测、PCR及测序、RT-PCR对缺陷菌进行鉴定。结果在含有红霉素(10μg/mL)的琼脂平板上出现转化菌落,鉴定结果均显示转化菌为comD缺陷菌。结论成功构建了变形链球菌UA159comD基因缺陷菌株。
Objective To construct the comD gene-deficient strain associated with the density-sensitive UAl59 strain of Streptococcus mutans, in preparation for further study on the function of this gene. Methods According to the principle of homologous recombination, transformed colonies were obtained by electroporation transformation using the homologous recombined DNA fragment of UAl59comD gene of Streptococcus mutans, and the defective colonies were identified by morphological observation, biochemical characterization, PCR, sequencing and RT-PCR. Results Transformed colonies appeared on agar plates containing erythromycin (10 μg / mL). The results showed that the transformed bacteria were comD-deficient bacteria. Conclusion The strain of Streptococcus mutans UA159comD gene was successfully constructed.