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目的:研究骨髓单个核细胞移植对实验性扩张型心肌病慢性心力衰竭大鼠心肌的修复作用及其远期效果。方法:建立扩张型心肌病慢性心力衰竭大鼠模型。造模后实验组直接心肌注射DAP I标记过的骨髓单个核细胞;对照组注射同等剂量PBS溶液;假手术组仅开胸和关胸。于造模后即刻、移植后4、12和24周,分批分组进行免疫组织化学和透射电镜等的检测分析。结果:心脏标本切片:荧光显微镜检查证实在细胞移植区有新生心肌和血管,因细胞核被标记而呈现荧光。透射电镜检查可见不成熟的心肌细胞,胞质中有散在肌丝样物质,还可见内膜不完整的新生血管,阳性物质随时间推移而逐渐增多。对照组和假手术组没有上述改变,并在不同时间段发生死亡病例。结论:骨髓单个核细胞移植能有效防止慢性心力衰竭大鼠心功能恶化,促进心肌和血管再生,有潜在治疗价值。
OBJECTIVE: To study the repairing effect and long-term effect of bone marrow mononuclear cell transplantation on myocardium of chronic heart failure rats with experimental dilated cardiomyopathy. Methods: To establish a rat model of chronic heart failure with dilated cardiomyopathy. After modeling, the experimental group received intramyocardial injection of DAP I-labeled bone marrow mononuclear cells directly; the control group received the same dose of PBS solution; the sham-operated group received only thoracotomy and closed chest. Immediately after modeling, at 4, 12 and 24 weeks after transplantation, immunohistochemistry and transmission electron microscopy were performed in batches for analysis. RESULTS: Cardiac sectioning: Fluorescence microscopy confirmed the presence of new-born myocardium and blood vessels in the cell-grafted area, as the nuclei were labeled and fluoresced. Transmission electron microscopy revealed immature cardiomyocytes, scattered in the cytoplasm myofilaments, but also shows incomplete neovascularization of the intima, positive substances gradually increased over time. The control group and sham group did not have the above changes, and deaths occurred at different time points. CONCLUSION: Bone marrow mononuclear cell transplantation can effectively prevent the deterioration of cardiac function and promote the regeneration of myocardium and blood vessels in rats with chronic heart failure, and has potential therapeutic value.