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[Objective] This study aimed to clone the PsSFBB gene from Xinjiang pear for bioinformatics analysis.[Method] PsSFBB gene was cloned from anthers of Qipan pear by using RT-PCR and RACE technologies for bioinformatics analysis.[Result] A SFBB6-α gene with a full-length of 1 231 bp was cloned and named PsSFBB6-α(Genbank accession number:EU909685).PsSFBB6-α gene encodes a protein of 378 amino acids,with an F-box motif composed of about 50 amino acids in the N-terminal.According to the bioinformatics analysis,the molecular formula of PsSFBB6-α protein is C2000H3034N517O558S23,with relative molecular mass of 43 987.5 and isoelectric point of 6.02,and the secondary structure is dominated by α-helices;theoretically,the half life period is 30 h and the instability parameter is 55.21,so PsSFBB6-α protein is an instable protein;in addition,it is predicted that PsSFBB6-α protein is a hydrophilic and non-secreted protein with lyases activity and specifically recognized substrates,which was consistent with the function of F-box protein.[Conclusion] This study laid the foundation for further research on SFBB proteins and the mechanism of self-incompatibility and provided theoretical basis for breeding of self-compatible cultivars of Xinjiang pear and scientific arrangement of pollination trees in production to increase the yield and quality.
[Objective] This study aimed to clone the PsSFBB gene from Xinjiang pear for bioinformatics analysis. [Method] PsSFBB gene was cloned from anthers of Qipan pear by using RT-PCR and RACE technologies for bioinformatics analysis. [Result] A SFBB6-α gene with a full-length of 1 231 bp was cloned and named PsSFBB6-α (Genbank accession number: EU909685) .PsSFBB6-α gene encodes a protein of 378 amino acids, with an F-box motif composed of about 50 amino acids in the N-terminal. According to the bioinformatics analysis, the molecular formula of PsSFBB6-α protein is C2000H3034N517O558S23, with a relative molecular mass of 43 987.5 and isoelectric point of 6.02, and the secondary structure is dominated by α-helices; theoretically, the half life period is 30 h and the instability parameter is 55.21, so PsSFBB6-alpha protein is an instable protein; in addition, it is predicted that PsSFBB6-alpha protein is a hydrophilic and non-secreted protein with lyases activity and specifically recognized substrates, which wasconsistent with the function of F-box protein. [Conclusion] This study laid the foundation for further research on SFBB proteins and the mechanism of self-incompatibility and provides theoretical basis for breeding of self-compatible cultivars of Xinjiang pear and scientific arrangement of pollination trees in production to increase the yield and quality.