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目的:探讨大黄素(emodin)联合吉西他滨(gemcitabine)对人胰腺癌细胞株Panc-1的增殖抑制作用及大黄素联合吉西他滨的最佳时机。方法:人胰腺癌细胞株Panc-1经大黄素10,20,40,80,160μmol·L-1分别作用24,48,72 h;以及在吉西他滨20μmol·L-1作用前24 h,0 h,后24,48,72 h分别联合大黄素20μmol·L-1作用24 h。应用Cell Counting Kit-8(CCK-8)法检测细胞增殖;镜下观察细胞形态学改变;流式细胞术检测细胞凋亡。结果:大黄素对Panc-1细胞的增殖抑制作用呈明显浓度和时间依赖性;大黄素单独或联合吉西他滨可显著诱导胰腺癌细胞凋亡;大黄素在吉西他滨作用前24,0 h,后24,48,72 h加入联合作用24 h后胰腺癌Panc-1细胞的生长抑制率分别为54.13%,49.58%,48.72%,40.74%,44.16%,大黄素作用吉西他滨前24 h组与其他各组相比较,均有统计学意义(P<0.05)。结论:大黄素可显著抑制人胰腺癌Panc-1细胞生长,大黄素可增强吉西他滨对人胰腺癌Panc-1细胞的增殖抑制和诱导凋亡作用,大黄素联合吉西他滨的最佳时机可能是在吉西他滨作用前24 h加入。
Objective: To investigate the inhibitory effect of emodin combined with gemcitabine on the proliferation of human pancreatic cancer cell line Panc-1 and the optimal timing of emodin combined with gemcitabine. Methods: Human pancreatic cancer cell line Panc-1 was treated with 10, 20, 40, 80 and 160μmol·L-1 emodin for 24,48,72 h, respectively. The cells were treated with 20 μmol·L-1 gemcitabine for 24 h, 24 hours, 48 hours and 72 hours after combined with emodin 20μmol·L-1 for 24 hours. Cell Counting Kit-8 (CCK-8) method was used to detect cell proliferation; morphological changes were observed microscopically; apoptosis was detected by flow cytometry. Results: Emodin significantly inhibited the proliferation of Panc-1 cells in a time-and concentration-dependent manner. Emodin alone or in combination with gemcitabine induced apoptosis of pancreatic cancer cells. Emodin significantly inhibited pancreatic cancer cell apoptosis at 24, The growth inhibition rates of pancreatic cancer Panc-1 cells after 48 h and 72 h treatment were 54.13%, 49.58%, 48.72%, 40.74% and 44.16%, respectively. The effect of emodin on the growth of Panc- Compared with statistical significance (P <0.05). Conclusion: Emodin can significantly inhibit the growth of Panc-1 cells in human pancreatic cancer. Emodin can enhance the inhibitory effect of gemcitabine on Panc-1 cells and induce the apoptosis of Panc-1 cells. Emodin and gemcitabine may be the best choice for gemcitabine 24 h before adding.