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目的探索即刻刺激对中国树鼩体内DA、cAMP、cGMP水平的影响。方法给予中国树鼩电刺激后,即刻采取血液和组织,用放射免疫法检测多巴胺(DA)、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)的水平。结果电刺激组中国树鼩大脑前额叶皮层(PFC)、海马(Hipp)、纹状体(striatum)、伏隔核(Nac)组织中DA水平比对照组分别升高了52.33%、38.98%、25.25%、45.06%(P<0.01);电刺激组中国树鼩血液中c AMP、c GMP水平比对照组分别增加了30.99%、14.94%(P<0.05,P<0.01);c AMP/c GMP比值比对照组增加了18.73%(P<0.01);电刺激组中国树鼩中脑腹侧背盖区(VTA)、大脑前额叶皮层(PFC)、海马(Hipp)、睾丸(Testicle)组织中c AMP水平比对照组分别升高了21.84%、24.55%、21.07%、44.87%(P<0.01),c GMP水平比对照组分别升高了17.95%、25.57%、35.33%、22.87%(P<0.05,P<0.01)。结论即刻刺激能促进中国树鼩体内DA、c AMP、c GMP的合成和释放。
Objective To explore the effect of immediate stimulation on the levels of DA, cAMP and cGMP in tree shrews in China. Methods The blood and tissues were taken immediately after the electrical stimulation of the tree shrews in China. The levels of dopamine (DA), cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were measured by radioimmunoassay. Results Compared with the control group, the levels of DA in electrical stimulation group increased by 52.33% and 38.98%, respectively, in the forehead cortex (PFC), hippocampal (Hipp), striatum and nucleus accumbens (Nac) 25.25% and 45.06%, respectively (P <0.01). The levels of cAMP and c GMP in the Chinese tree 鼩 blood were increased by 30.99% and 14.94% (P <0.05, P <0.01) GMP ratio increased 18.73% (P <0.01) compared with the control group. In the electro-acupuncture group, the ventral tegmental area (VTA), the prefrontal cortex (PFC), the hippocampus, the testicle tissue The levels of c-AMP increased by 21.84%, 24.55%, 21.07%, 44.87% (P <0.01), and the levels of c-GMP increased by 17.95%, 25.57%, 35.33%, 22.87% P <0.05, P <0.01). Conclusion Immediate stimulation can promote the synthesis and release of DA, c AMP and c GMP in Chinese tree trunks.