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分别用仙客来种苗的子叶和叶柄作为外植体,培养在附加2,4 D、细胞分裂素和细胞激动素的1/2MS培养基上诱导不定芽的发生,研究了愈伤组织和不定芽形成过程中的组织细胞学变化.对两种不同外植体不定芽发生过程中的切片观察表明,两种外植体的组织脱分化始于维管束周围的维管束鞘细胞,随后开始分裂的是与其临近的薄壁细胞并能很快形成胚性分生细胞团.经30d左右的培养,进一步分化形成芽原基.子叶愈伤的芽原基通常出现在愈伤的边缘.叶柄脱分化比子叶快,不定芽可以起源于表层的分生细胞团,也可以由愈伤深处的分生组织分化而形成.
The cotyledons and petioles of cyclamen seedlings were used as explants to induce adventitious buds on 1/2 MS medium supplemented with 2,4 D, cytokinin and cytokinin, respectively. The effects of callus and Histological changes during adventitious bud formation. Observation of the sections of adventitious buds from two different explants showed that dendifferentiation of the two explants began with vascular bundle sheath cells around the vascular bundle, and then started Divided with its adjacent parenchyma cells and the rapid formation of embryogenic meristematic cells.After about 30d culture, further differentiation to form bud primordia.Cotyledon callus bud primordia usually appear in the edge of the callus. Petiole Dedifferentiation than cotyledons, adventitious buds can originate from the surface meristematic cell mass, but also by the callus of meristem differentiation.