新疆啤酒花潜隐病毒的检测及外壳蛋白基因的序列分析

• 作 者: 刘升学 韩盛 祝建波 向本春 曹连莆
• 机 构: 新疆生产建设兵团绿洲生态农业重点实验室,石河子大学农学院,新疆生产建设兵团绿洲生态农业重点实验室,新疆生产建设兵团绿洲生态农业重点实验室,新疆生产建设兵团绿洲生态农业重点实验室 石河子832003,石
• 出 处: 植物病理学报
• 发表日期: 2008年03期
• 关键词: 新疆 啤酒花品种 病毒 检测 外壳蛋白基因 序列分析 latent virus 寄主范围 种植区 症状 危害 感染 分布

Hop samples from different region in Xinjiang were collected and detected by reverse transcription-polymerase chain reaction(RT-PCR) in order to determine the occurrence of HpLV.The RT-PCR products were 314 bp and 964 bp.Coat protein(CP) gene segments of isolates AW3 from Wensu,CH1 from Changji,K6 from Yanqi,SW8 from Shawan,TE2 from Emin,TY4 from Tacheng and YL4 from Yili were cloned.Nucleic acid sequence of the CP gene and deduced CP amino acid sequence alignments were compared with AB032469 registered in GenBank.The result showed that 8 HpLV isolates were divided into two groups.Among the isolates,AB032469,K6,AW3,CH1,TE2,TY4 and YL4 were classified as GroupⅠand SW8 was assigned to GroupⅡ.GroupⅠisolates fell into three small clusters.Nucleotide and deduced amino acid sequence comparisons within GroupⅠisolates showed that the sequence homologies were 98.1%-99.9% and 99.0%-100%,respectively. Comparisons of nucleotide and deduced amino acid sequences between Group Ⅰ and Group Ⅱ showed that the sequence homologies were 95.8%-96.4% and 98.4%-99.0%,respectively.Amino acid identity was higher than nucleic acid identity among all isolates.

Hop samples from different regions in Xinjiang were collected and detected by reverse transcription-polymerase chain reaction (RT-PCR) in order to determine the occurrence of HpLV. The RT-PCR products were 314 bp and 964 bp. Coat protein (CP) gene segments of isolates AW3 from Wensu, CH1 from Changji, K6 from Yanqi, SW8 from Shawan, TE2 from Emin, TY4 from Tacheng and YL4 from Yili were cloned. Nucleic acid sequence of the CP gene and deduced CP amino acid sequence alignments were compared with AB032469 registered in GenBank. The result showed that 8 HpLV isolates were divided into two groups. Among the isolates, AB032469, K6, AW3, CH1, TE2, TY4 and YL4 were classified as Group I and SW8 was assigned to Group II. Group I isolates fell into three small clusters. Nucleotide and deduced amino acid sequence comparisons within Group I isolates showed that the sequence homologies were 98.1% -99.9% and 99.0% -100%, respectively. Comparisons of nucleotide and deduced amino acid sequences between Group I and Group showed that the sequence homologies were 95.8% -96.4% and 98.4% -99.0%, respectively.Amino acid identity was higher than nucleic acid identity among all isolates.