论文部分内容阅读
本研究旨在通过研究EB病毒(EBV)感染对脐带血CD4+CD25+调节性T细胞(Treg)Foxp3表达的影响,进一步揭示EBV逃逸宿主免疫监视的机制。将重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)和重组人白细胞介素4(rhIL-4)诱导的脐带血单核细胞来源的DC与自体混合淋巴细胞进行共培养,流式细胞术检测共培养体系中CD4+CD25highT细胞的比例及CD4+CD25+T细胞Foxp3的表达。在DC与混合淋巴细胞共培养比率为1∶10时,病毒感染组CD4+CD25+T细胞Foxp3表达明显高于对照组;在共培养比率为1∶1时,与EBV感染的DC共培养的混合淋巴细胞中CD4+CD25highT细胞的百分比明显高于LPS诱导成熟的DC,差异均具有统计学意义。提示EBV感染的脐带血单核细胞来源的DC通过上调Treg分化和发育因子Foxp3而诱导了自体Treg的活化,这可能是EBV逃逸宿主免疫监视的机制之一。
The aim of this study was to investigate the mechanism of immune surveillance of EBV escape host by studying the effect of Epstein-Barr virus (EBV) infection on Foxp3 expression in cord blood CD4 + CD25 + regulatory T cells (Tregs). DCs derived from cord blood mononuclear cells induced by recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and recombinant human interleukin-4 (rhIL-4) were co-cultured with autologous mixed lymphocytes, The percentage of CD4 + CD25highT cells in co-culture system and the expression of Foxp3 in CD4 + CD25 + T cells were detected by cytometry. Foxp3 expression of CD4 + CD25 + T cells in virus-infected group was significantly higher than that in control group when the co-culture ratio of DCs and mixed lymphocytes was 1:10. When the co-culture ratio was 1: 1, co-cultured with EBV-infected DCs The percentage of CD4 + CD25highT cells in mixed lymphocytes was significantly higher than that of DCs induced by LPS, the differences were statistically significant. It is suggested that EBV-infected cord blood mononuclear cell-derived DCs induce autologous Treg activation by upregulating Treg differentiation and developmental factor Foxp3, which may be one of the mechanisms of EBV escape host immune surveillance.