小鼠下颔下腺中肥大细胞异质性研究

• 作 者: 贾雪梅 贾友苏 齐威琴 王惠珠
• 机 构: 安徽医科大学组织胚胎学教研室,安徽医科大学组织胚胎学教研室,安徽医科大学组织胚胎学教研室,安徽医科大学组织胚胎学教研室 合肥230032,合肥230032,合肥230032,合肥230032
• 出 处: 解剖学杂志
• 发表日期: 1996年04期
• 关键词: 肥大细胞 异质性 下颌下腺 甲苯胺蓝染色 小鼠 免疫反应 小叶间导管 腺实质 免疫组织化学 神经元胞体

取小鼠下颌下腺,用甲苯胺蓝染色、Alcian蓝一藏红染色及免疫组织化学ABC法显示肥大细胞的异质性.结果表明;肥大细胞主要分布于间质的小血管、小叶间导管及神经节周围.此外还发现有些肥大细胞沿腺实质表面排列,有些肥大细胞伸出突起与相邻的神经元或肥大细胞接触.肥大细胞呈降钙素基因相关肽免疫反应阳性,但仅为相邻切片甲苯胺蓝染色的14 %.提示下颌下腺中肥大细胞的存在可能与腺体分泌活动及血流调节有关.

The submandibular glands of mice were taken out, and the heterogeneity of mast cells was observed by toluidine blue staining, Alcian blue-hid dye staining and immunohistochemical ABC method.The results showed that mast cells were mainly distributed in interstitial small vessels, interlobular ducts, Ganglion around. In addition, it was also found that some mast cells arranged along the surface of the glandular parenchyma, some mast cells protruding process and adjacent neurons or mast cells contact mast cells were positive for calcitonin gene-related peptide immunoreactivity, but only for the phase Adjacent sections toluidine blue stained 14%, suggesting that the presence of mast cells in the submandibular gland may be related to glandular secretion activity and blood flow regulation.