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通过花粉管通道法将受照射的中 5DNA(小偃麦异源 8倍体 ,抗大麦黄矮病 )导入到受体小麦新克旱 9,在D3～D5 代获得了稳定的 5 4个品系。对其籽粒醇溶蛋白和麦谷蛋白进行电泳出现 3种变异类型 :第 1种类型为新谱带的出现或原有谱带的丢失 ;第 2种类型为供体谱带的出现 ;第 3种类型为谱带强度 (深或浅 )发生变化。根据农艺性状和籽粒贮藏蛋白电泳结果 ,精选 2 0份进行毒蚜接种 ,鉴定大麦黄矮病抗性 ,其中有 7个品系中抗黄矮病 ;1个品系 (97K1 0 77)高抗黄矮病。可初步认为供体中 5的抗大麦黄矮病基因已转化成功
The irradiated middle 5DNA (alloploid hybrids of O. tritici, anti-barley yellow dwarf virus) was introduced into the new wheat cultivar Xinkan9 via pollen tube pathway, and 54 stable strains were obtained from D3 to D5 . There were three types of variation in the electrophoresis of grain prolamins and glutenin: the first type was the appearance of a new band or the loss of the original band; the second type was the appearance of a donor band; the third type Change the intensity (dark or light) of the band. According to agronomic traits and grain storage protein electrophoresis results, twenty (20) were selected for inoculation with aphids to identify barley yellow dwarf virus resistance, of which seven were resistant to yellowish dwarf disease. One strain (97K1 077) Dwarf disease. It can be preliminarily thought that the anti-barley yellow dwarf virus gene from donor 5 has been transformed successfully