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目的制备不同分子量范围的麦冬寡糖,考察其体外抗α-葡萄糖苷酶活性,探究麦冬寡糖的分子量与抗α-葡萄糖苷酶活性的关系。方法采用乙醇和水提取麦冬,以Sephadex G-75柱层析法进行脱色脱蛋白,以Sephadex G-15柱层析法对麦冬寡糖进行分级分离。利用基质辅助激光解析飞行质谱仪(MALDI-TOF-MASS)检测其分子量范围;并采用来源于啤酒酵母的α-葡萄糖苷酶和来源于大鼠的具有α-葡萄糖苷酶活性的小肠绒毛提取物测定各麦冬寡糖的抑制α-葡萄糖苷酶活性。结果采用Sephadex G-15柱层析法对麦冬寡糖进行分级分离,得到两种不同分子量的麦冬寡糖组分,经MALDI-TOF-MASS检测,其分子量范围分别为1100~2500(MDPS-1)和500~1500(MDPS-2)。该两部分麦冬寡糖组分对啤酒酵母来源的α-葡萄糖苷酶和来源于大鼠小肠绒毛提取物的α-葡萄糖苷酶均有明显的抑制作用,且在50~200 mg/m L的浓度范围内呈一定量效关系。其中分子量范围为500~1 500的麦冬寡糖组分的抑制作用更强。结论麦冬寡糖的分子量与其抗α-葡萄糖苷酶活性有密切关系,分子量范围为500~1 500的麦冬寡糖具有更显著抑制α-葡萄糖苷酶活性,为重要的功效组分。
OBJECTIVE To prepare the oligodextrins with different molecular weight and investigate the anti-α-glucosidase activity in vitro and to explore the relationship between the molecular weight and the anti-α-glucosidase activity. Methods Ophiopogon japonicus was extracted with ethanol and water, and then deproteinized by Sephadex G-75 column chromatography. Osthole oligosaccharides were fractionated by Sephadex G-15 column chromatography. The molecular weight range was determined by using MALDI-TOF-MASS; α-glucosidase derived from Saccharomyces cerevisiae and intestinal villus extract derived from rat with α-glucosidase activity The inhibitory alpha-glucosidase activity of each of the Orthogonal Tests was determined. Results Osthole oligosaccharides were fractionated by Sephadex G-15 column chromatography to obtain two kinds of oligosaccharides with different molecular weights. Their molecular weights ranged from 1100 to 2500 (MDPS -1) and 500 to 1500 (MDPS-2). The two parts of Ophiopogon oligosaccharides components of beer yeast derived α-glucosidase and derived from rat intestinal flu extract α-glucosidase were significantly inhibited, and in the 50 ~ 200 mg / m L The concentration range of a certain amount of effective relationship. Among them, the inhibitory effect of the components of Ophiopogon oligosaccharides with the molecular weight range of 500-1,500 was stronger. Conclusion The molecular weight of Ophiopogon oligosaccharides is closely related to its anti-α-glucosidase activity. Oosalose oligosaccharides with a molecular weight range of 500-1,500 have more significant inhibitory effects on α-glucosidase activity, which is an important functional component.