论文部分内容阅读
目的 探讨癫痫过程中神经细胞Na+ 、K+ 通透性的变化。方法 应用Na+ 、K+ 选择性微电极检测了马桑内酯 (5 μl,5× 10 -4 mol/L)灌流的大鼠海马脑片神经细胞外Na+ 、K+ 活度的改变。结果 马桑内酯灌流海马脑片 30s后可观察到海马神经细胞外Na+ 活度明显下降 ,与对照组比较下降幅度为 10mmol/L(P <0 .0 1) ,在实验观察的时间内 (2 0min)未见Na+ 活度的恢复 ;海马神经细胞外K+ 活度在灌流后 1min、2min、3min、5min和 10min分别高于对照组 3.3mmol/L、4.8mmol/L、4.9mmol/L、6 .5mmol/L和 8.8mmol/L ,在以后 10min内K+ 活度亦未见恢复。结论 马桑内酯作用于离体海马神经细胞时存在Na+ 内流、K+ 外流现象
Objective To investigate the changes of Na + and K + permeability of nerve cells during epilepsy. Methods The changes of Na + and K + activity in hippocampal slices of rats perfusing with 2% (5 μl, 5 × 10 -4 mol / L) of corundams were detected by Na +, K + selective microelectrodes. Results The intracellular Na + activity of hippocampal neurons decreased significantly after 30s of intramuscular lactone perfusion in hippocampal slices. Compared with the control group, the decrease of Na + activity was 10mmol / L (P <0.01) 2mmol / L, 4.8mmol / L and 4.9mmol / L respectively in control group at 1min, 2min, 3min, 5min and 10min after perfusion. 6 .5mmol / L and 8.8mmol / L, in the next 10min K + activity also did not recover. CONCLUSIONS: Coronasol can induce Na + influx and K + efflux in hippocampal neurons in vitro