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探讨Sm(NO3)3慢性暴露对大鼠血流动力学、心脏组织结构及血常规指标的影响。SD大鼠48只,雌雄各半,随机分成4组,分别自由饮用0,3,4.5,6 mg·L-1Sm(NO3)3溶液180 d。开胸取心时采血进行血常规测定,心脏光镜切片观察心肌组织形态学的变化;部分心脏离体后应用Langendorff离体心脏灌流模型,观察Sm(NO3)3慢性暴露对大鼠心脏功能的影响。结果显示3 mg·L-1组LVSP,+dp/dtmax极显著地增高(p<0.01),CF显著下降(p<0.05);但4.5,6 mg·L-1组LVSP显著增高(p<0.05),LVEDP,+dp/dtmax,-dp/dtmax,HR,CF等指标无明显改变;3,4.5 mg·L-1Sm(NO3)3组WBC,MID,Lym数与对照相比有极显著和显著增高(p<0.01,p<0.05)。3 mg·L-1组心肌充血出血,肌束呈波浪状,肌细胞体积增大,胞浆内呈红染的颗粒状变性,核轻度增大。4.5 mg·L-1组心肌内皮细胞增生,小灶肌束断裂,部分细胞颗粒状变性。6 mg·L-1组心肌肌束排列紊乱,微灶细胞变性坏死,细胞核消失,间质少量淋巴细胞浸润。以上结果表明一定浓度Sm(NO3)3对离体大鼠心脏有正性变力作用;增加WBC,MID,Lym数,并可导致心肌组织的代偿性增生与损伤。
To investigate the effects of chronic exposure to Sm (NO3) 3 on hemodynamics, cardiac tissue and blood parameters in rats. Forty-eight SD rats were randomly divided into 4 groups according to sex, male and female, respectively, and were given 0, 3, 4.5, 6 mg · L-1Sm (NO3) 3 solution for 180 days. Thoracotomy and blood sampling were performed to determine the changes of myocardial histopathology. Langendorff perfusion was used to observe the changes of myocardial tissue morphology in some hearts. The effects of chronic exposure to Sm (NO3) 3 on cardiac function influences. The results showed that the LVSP, + dp / dtmax increased significantly (p <0.01), CF decreased significantly (p <0.05) in 3 mg · L-1 group, while the LVSP increased significantly in 4.5,6 mg · L-1 group 0.05). The indexes of LVEDP, dp / dtmax, -dp / dtmax, HR and CF did not change significantly. The numbers of WBC, MID and Lym in 3,4.5 mg · L-1Sm (NO3) And significantly higher (p <0.01, p <0.05). 3 mg · L-1 group of myocardial congestion and bleeding, muscle bundles were wavy, muscle cell volume increased, the cytoplasm of red staining of granular degeneration, a slight increase in nuclear. 4.5 mg · L-1 group of myocardial endothelial cell proliferation, small muscle bundle fracture, granular degeneration of some cells. In the 6 mg · L-1 group, the myocardial muscle bundles were disordered, the neutrophils were degenerated and necrotic, the nuclei disappeared and the interstitial few lymphocytes infiltrated. The above results show that certain concentrations of Sm (NO3) 3 have a positive inotropic effect on the isolated rat heart; increase WBC, MID, and Lym number, and can lead to compensatory hyperplasia and injury of myocardial tissue.