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目的探讨3q21.3内常见变异rs10934853单核苷酸多态性与中国人前列腺癌发病风险的关系,并探讨其与临床相关危险因素的相互作用。方法应用病例对照方法,选取90例前列腺癌患者以及年龄、性别匹配的109例正常对照人群为研究对象,采用聚合酶链反应-高分辨熔解曲线(PCR-HRM)技术结合测序验证法检测其3q21.3内rs10934853单核苷酸多态性的分布情况。结果①前列腺癌组中AA,CC,AC基因型分别为19例(21.1%),34例(37.8%),37例(41.1%);正常对照组中AA,CC,AC基因型分别为24例(22.0%),34例(31.2%),51例(46.8%);两组间基因型频率和等位基因频率的分布无显著性差异(P>0.05)。②Rs10934853单核苷酸多态性的不同基因型与前列腺癌患者的年龄,BMI指数,Gleason评分以及PSA浓度指标间均无显著相关性(P>0.05)。结论 Rs10934853单核苷酸多态性与中国人前列腺癌的发生无明显相关性,可能与中国人前列腺癌发病风险无关。
Objective To investigate the relationship between the common mutation rs10934853 SNP in 3q21.3 and the risk of prostate cancer in Chinese and its interaction with clinically relevant risk factors. Methods Ninety patients with prostate cancer and 109 normal control subjects of age and sex matched for the study were selected as case-control. PCR-HRM and sequencing were used to detect the expression of 3q21 .3 Distribution of rs10934853 SNPs. Results ① The genotypes of AA, CC and AC in prostate cancer group were 19 (21.1%), 34 (37.8%) and 37 (41.1%) respectively. The AA, CC and AC genotypes in the control group were 24 (22.0%), 34 cases (31.2%) and 51 cases (46.8%). There was no significant difference in genotype frequency and allele frequency between the two groups (P> 0.05). ② There was no significant correlation between different genotypes of rs10934853 SNP and age, BMI, Gleason score and PSA concentration in patients with prostate cancer (P> 0.05). Conclusion There is no significant correlation between single nucleotide polymorphism of Rs10934853 and the occurrence of prostate cancer in Chinese, which may not be related to the risk of prostate cancer in Chinese.