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硝苯地平(Ⅰ)是一种易光解的钙拮抗剂。在儿科病人中,由于Ⅰ的片剂不易吞咽,一般将其研成粉末后给药,因此有必要检测Ⅰ粉末在室内光照条件下的稳定性。本文建立了一种采用两个内标来测定医院处方中Ⅰ及其光解产物(Ⅱ)的HPLC法。色谱条件采用固定相为5μm Develosil ODS-5的填充柱(150×4.6 mm,id),流动相为0.01 mol/L磷酸氢二钠缓冲液-甲醇(45:55,ν/ν),混合前用50％的磷酸调pH至6.1,流速1.0 ml/min,检测波长254
Nifedipine (I) is a readily photolytic calcium antagonist. In pediatric patients, because I tablets are not easily swallowed, they are usually powdered and administered. It is therefore necessary to test the stability of I powder under indoor lighting conditions. In this paper, an HPLC method for the determination of Ⅰ and its photolysis products (Ⅱ) in hospitals using two internal standards was established. The chromatographic conditions were as follows: a packed column (150 × 4.6 mm, id) of Develosil ODS-5 with a stationary phase of 5 μm and a mobile phase of 0.01 mol / L dibasic sodium phosphate dibasic buffer methanol (45:55, Adjust pH to 6.1 with 50% phosphoric acid, flow rate 1.0 ml / min, detection wavelength 254