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生药的正确鉴定,不但是天然药物进行化学和药理研究的前提条件,而且也是临床用药的根本保障。运用以DNA为基础的多形态分析鉴定生药,比单纯的形念分析和化学鉴定有如下优点:①表现型和基因型都是直接分析,因此结果不受环境因素的影响。②分析中可选择一个或多个具有一定进化比率的基因,解决一些特殊的问题。③所需样品数量很少,尤其分析中应用PCR(聚合酶链反应),则所需量更少。在大量存在酚类和(或)多糖类化合物溶液中,很难将生药的DNA提纯出来,各种难度较大的步骤必须结合在一起以获取
The correct identification of crude drugs is not only a prerequisite for natural medicines for chemical and pharmacological research, but also a fundamental guarantee for clinical drug use. The use of DNA-based polymorphic analysis to identify crude drugs has the following advantages over simple shape analysis and chemical identification: 1 Both phenotypes and genotypes are directly analyzed, so the results are not affected by environmental factors. 2 The analysis can select one or more genes with a certain evolutionary ratio to solve some special problems. 3 The number of required samples is small, especially in the analysis using PCR (polymerase chain reaction). In the presence of a large amount of phenolic and/or polysaccharide compounds, it is difficult to purify the crude drug DNA. Various difficult steps must be combined to obtain