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我们建立了一种半套式反转录ＰＣＲ检测水中肠道病毒的核酸，即采用一对半（三条）引物进行两次ＰＣＲ扩增，其敏感性可检测只含１个ｐｆｕ病毒的水样。本方法特异性强、敏感性高，可避免由于环境污染造成的假阳性结果；成本相对较低。
We have developed a semi-nested reverse-transcription PCR for the detection of enteric viruses in water using two pairs of primers (three pairs) for two PCR amplifications that are sensitive to detect water samples containing only one pfu virus . The method has the advantages of high specificity and high sensitivity, and can avoid the false positive result caused by environmental pollution; and the cost is relatively low.