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目的:采用同种和异种睾丸组织移植的方法,研究新生小鼠睾丸组织及人类未成熟睾丸组织异种移植物在免疫缺陷小鼠体内发育不同时期生精细胞的组成和基因表达情况中生精细胞的发育情况。方法:以免疫缺陷小鼠为受体,新生小鼠睾丸组织和人类未成熟睾丸组织为供体,分别进行同种和异种移植。通过对移植物的组织形态学观察和分子生物学检测,对各个时期同种移植物中的生精细胞组成及其特异性基因的表达情况进行评估并与末受损小鼠的情况相比较;对人睾丸组织异种移植物的存活及其生精细胞在异体异位的发育情况进行探讨。结果:新生小鼠睾丸组织在成年雄性去势免疫缺陷小鼠体内的发育状况在移植开始的一个阶段与在体睾丸组织的发育情况基本相同,各级生精细胞的出现及其基因表达均与在体睾丸组织中相类似,而移植7-8星期后生精小管发生退化现象。人未成熟睾丸组织在受体中存活并且进一步生长;组织学观察还发现,生精细胞的发育速度与在体相比具有加速的倾向。结论:新生小鼠睾丸组织同种移植物的发育与在体情况基本相同,而人类未成熟睾丸组织异种移植物的发育与正常生理状态相比较呈现出加速的倾向。
OBJECTIVE: To study the composition and gene expression of spermatogenic cells in different developmental stages of immunodeficient mice using testicular tissue and human immature testicular tissue xenografts of allogeneic and heterologous testicular tissue transplantation. The development of the situation. Methods: The immunodeficient mice were used as recipients and the newborn mice testis and human immature testis were used as donors. Through the observation of histomorphology and molecular biology, the composition of spermatogenic cells and the expression of specific genes in the allografts in different periods were evaluated and compared with those in the mice with no damage at all. Survival of human testicular tissue xenografts and the development of their spermatogenic cells in allogeneic ectopic were explored. Results: The development of newborn mouse testes in adult male castrated mice was basically the same as the in vivo testes in one stage of transplantation. The appearance of spermatogenic cells and their gene expression at all levels were correlated with In somatic testis tissue similar, and 7-8 weeks after transplantation, the seminiferous tubules degeneration. Human immature testicular tissue survives the recipient and grows further; histological observations also show that spermatogenic cells tend to develop at a faster rate than in vivo. CONCLUSION: The development of allograft in testis tissue of neonatal mice is basically the same as that in vivo. However, the development of xenografts in human immature testis tissue tends to be accelerated compared with the normal physiological state.