论文部分内容阅读
AIM:To construct a pEgr-IFNy plasmid and to investigateits expression properties of interferon-γ(INF-γ)induced byirradiation and the effect of gene-radiotherapy on thegrowth of melanoma.METHODS:A recombined plasmid,pEgr-IFNγ,wasconstructed and transfected into B16 cell line withlipofectamine.The expression properties of pEgr-IFNγ wereinvestigated by ELISA.Then,a B16 melanoma-bearingmodel was established in mice,and the plasmid wasinjected into the tumor tissue.The tumor received 20 GyX-ray irradiation 36 h after injection,and IFN-γ expressionwas detected from the tumor tissue.A tumor growth curveat different time points was determined.RESULTS:The eukaryotic expression vector,pEgr-IFNγ,was successfully constructed and transfected into B16 cells.IFN-γ,expression was significantly increased in transfectedcells after X-ray irradiation in comparison with 0 Gy group(77.73-94.60 pg/mL,P<0.05-0.001),and was significantlyhigher at 4 h and 6 h than that of control group after 2 GyX-ray irradiation(78.90-90.00 pg/mL,P<0.01-0.001).When the transfected cells were given 2 Gy irradiation 5 timesat an interval of 24 h,IFN-γ expression decreased in atime-dependent manner.From d 3 to d 15 after IFNγ gene-radiotherapy,the tumor growth was significantly slowerthan that after irradiation or gene therapy alone.CONCLUSION:The anti-tumor effect of pEgr-IFNγ gene-radiotherapy is better than that of genetherapy or radiotherapyalone for melanoma.These results may establish an importantexperimental basis for gene-radiotherapy of cancer.
AIM: To construct a pEgr-IFNy plasmid and to investigate the expression properties of interferon-γ (INF-γ) induced by radiation and the effect of gene-radiotherapy on the growth of melanoma. METHODS: A recombined plasmid, pEgr-IFNγ, wasconstructed and transfected into B16 cell line withlipofectamine. The expression properties of pEgr-IFNγ were in examined by ELISA. Chen, a B16 melanoma-bearing model was established in mice, and the plasmid was injected into the tumor tissue. , and IFN-γ expression was detected from the tumor tissue. A tumor growth curveat different time points was determined .RESULTS: The eukaryotic expression vector, pEgr-IFNγ, was successfully constructed and transfected into B16 cells. IFN- γ expression was was increased in transfected cells after X-ray irradiation in comparison with 0 Gy group (77.73-94.60 pg / mL, P <0.05-0.001), and was significantlyhigher at 4 h and 6 h than that of control group after 2 GyX-ray ir radiation (78.90-90.00 pg / mL, P <0.01-0.001) .When the transfected cells were given 2 Gy irradiation 5 timesat an interval of 24 h, the IFN-γ expression decreased in a time-dependent manner. From d 3 to d 15 after IFNγ gene-radiotherapy, the tumor growth was significantly slowerthan that after irradiation or gene therapy alone. CONCLUSION: The anti-tumor effect of pEgr-IFNγ gene-radiotherapy is better than that of gene therapy or radiotherapyalone for melanoma. These results may establish an importantexperimental basis for gene-radiotherapy of cancer.