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蛋白质翻译后修饰(post-translational modifications,PTMs)在调节蛋白质的结构和功能上发挥着重要作用,异常的蛋白质翻译后修饰会导致某些疾病的发生.泛素化(ubiquitination)作为一类重要的蛋白质翻译后修饰,已经被证明与细胞的稳态以及细胞内部的多条信号通路有关,主要参与细胞内蛋白的定位、调节和降解以及细胞周期、基因表达、信号传递、损伤修复、炎症免疫等多种生命过程.然而,对于泛素蛋白的相关研究虽然进行了多年,但是许多重要的科学问题尚未研究清楚,其中如何高效地获得大量、均一的泛素化蛋白是最重要的挑战之一.近年来,蛋白质化学合成及半合成手段的发展,很大程度上解决了这一难题.本文主要针对目前常用的泛素化蛋白的化学合成和半合成方法进行综述.
Post-translational modifications (PTMs) play an important role in regulating the structure and function of proteins, and abnormal protein post-translational modifications lead to certain diseases. Ubiquitination is an important class Post-translational modification of proteins has been shown to be involved in the homeostasis of cells and multiple signaling pathways within cells, and is mainly involved in the localization, regulation and degradation of proteins in cells as well as in cell cycle, gene expression, signaling, injury repair, inflammatory immunity and the like However, many studies on ubiquitin have been carried out for many years, but many important scientific issues have not been studied yet. One of the most important challenges is how to efficiently and efficiently obtain large amounts of ubiquitinated proteins. In recent years, the development of protein chemical synthesis and semisynthetic means has largely solved this problem.In this paper, the chemical synthesis and semi-synthesis methods of ubiquitinated proteins are reviewed.