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应用反相高效液相色谱法，以大黄酸为定量指标，推断糖尿灵中结合蒽醌含量高低与泻下作用的关系。本法加样回收率为１０２．２４％，重现性试验ＲＳＤ＝３．５９％。此方法简便易行。
Reversed-phase high-performance liquid chromatography was used to determine rhein as a quantitative index to determine the relationship between the content of glycosides in glycosylation and the effect of diarrhea. The sample recovery rate was 102.24%, and the reproducibility test RSD was 3.59%. This method is simple and easy.