,Identification of SNPs involved in regulating a novel alternative transcript of P450 CYP6ER1 in the

• 作 者: Zhi-Kun Liang Rui Pang Yi Dong Zhong-Xiang Sun Yan Ling Wen-Qing Zhang
• 机 构: State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, China“,”Guangxi Academy of Ag
• 出 处: 中国昆虫科学（英文版）
• 发表日期: 2018年5期
• 关键词: alternative splicing cytochrome P450 Nilaparvata lugens single nucleotide polymo

Cytochrome P450-mediated metabolic resistance is one of the major mechanisms involved in insecticide resistance.Although the up-regulation of cytochrome P450 plays a vital role in insecticide metabolism,the molecular basis for the transcriptional regulation of cytochrome P450 remains largely unknown.The P450 gene CYP6ER1,has been reported to confer imidacloprid resistance to the brown planthopper,Nilaparvata lugens.Here,we identified a novel alteative transcript of CYP6ER1 (transcript A2) that had different expression pattes between resistant and susceptible populations,and was more stable after insecticide induction.The promoter of this transcript was sequenced and multiple single nucleotide polymorphisms (SNPs) were detected in individuals from susceptible and resistant field-collected populations.Resistant alleles of four SNPs were found to significantly enhance the promoter activity of the CYP6ER1 transcript A2.Electrophoretic mobility shift assays (EMSAs) revealed that these SNPs might regulate the binding of transcription factors to the promoter.Our findings provide novel evidence regarding the transcriptional regulation of a metabolic resistance-related gene and may be useful to understand the resistance mechanism of N.lugens in the field.