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利用钳位均匀电场脉冲电泳系统,从电泳的时间、角度、电压、转换时间间隔等方面优化电泳参数,确定了适宜拟青霉属内4种虫生真菌染色体DNA电泳的最佳条件。根据电泳及软件分析结果,估算出斜链拟青霉和环链拟青霉的染色体数目和染色体组型大小,即核型(karyotype)特征。通过进行拟青霉属内4种7株虫生真菌染色体DNA核型相关的比较和分析,表明拟青霉菌种间染色体数目差异较小,核型差异较大;相同菌种不同菌株间的染色体数、核型大小并不完全相同,存在不同程度的差异;且菌株间核型大小差异程度明显小于菌种间的差异程度。
The optimal electrophoresis parameters of four kinds of entomogenous fungi were analyzed by using the clamp uniform electric field pulse electrophoresis system in terms of electrophoresis time, angle, voltage and time interval. Based on the results of electrophoresis and software analysis, the chromosome numbers and the karyotypes of Paecilomyces parapertatus and Paecilomyces parahaemolyticus were estimated, ie karyotype characteristics. Through the comparison and analysis of chromosome DNA karyotypes of the four kinds of entomogenous fungi in Paecilomyces, the differences in the number of chromosomes between Paecilomyces species are small and the karyotypes are quite different. The chromosomes between different strains of the same species Number and karyotype size are not identical, there are different degrees of differences; and strains of karyotype size difference was significantly less than the degree of differences between strains.