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在粳稻品种武育粳3号栽培群体中获得一个类病斑突变体wy3。该突变体类病斑出现于苗期,分蘖期扩散至整张叶片,属于扩散型类病斑突变体。相比野生型,突变体wy3的株高明显降低,有效分蘖数减少,穗长、每穗粒数、结实率均显著降低。遮光处理表明,突变体wy3类病斑的产生受自然光诱导。台盼蓝染色结果表明,类病斑部位有大量的死亡细胞。突变体wy3的光合色素含量和净光合速率较野生型显著降低,SOD、POD、CAT活性和MDA含量均显著高于野生型。遗传分析表明突变体表型受单隐性核基因控制,采用BSA将该基因初步定位在第2染色体短臂端粒附近。采用F2群体中1099株类病斑单株将基因定位在标记W2-17和W2-18之间28kb的物理距离内。测序结果表明,突变体wy3中的LOC_Os02g02000编码区(CDS)第375位碱基C缺失,导致翻译提前终止,突变体中该候选基因为OsHPL3的一个新等位基因。
A lesion mutant wy3 was obtained from the cultivated population of japonica rice Wuyujing 3. The mutant appeared in seedling stage, tillering stage spread to the entire leaf, belonging to the proliferation of lesions like lesions. Compared with the wild type, the plant height of mutant wy3 decreased significantly, the number of effective tillers decreased, the ear length, the number of grains per spike, and the seed setting rate decreased significantly. Shading treatment showed that the generation of mutant wy3-like lesions was induced by natural light. Trypan blue staining results show that there are a large number of dead cells in the lesion-like area. The photosynthetic pigment content and net photosynthetic rate of mutant wy3 were significantly lower than that of wild type, and the activities of SOD, POD, CAT and MDA were significantly higher than those of wild type. Genetic analysis showed that the mutant phenotype was controlled by a recessive nuclear gene. The gene was initially located near the short arm telomere on chromosome 2 using BSA. A single strain of 1099 stains in the F2 population was used to locate the gene within a 28 kb physical distance between markers W2-17 and W2-18. The sequencing results showed that the deletion of the base C at position 375 of the LOC_Os02g02000 coding region (CDS) in the mutant wy3 resulted in the early termination of translation and the candidate gene in the mutant was a new allele of OsHPL3.