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从细胞分子水平，采用大脑皮层神经细胞分离技术，自旋标记技术，酶学方法及细胞染色技术探讨自由基对神经细胞的损伤及ＥＧｂ保护作用。结果显示，用自由基攻击的细胞其自旋标记膜的序参数Ｓ，旋转相关时间τｃ及膜蛋白巯基的Ｓ／Ｗ比对照组高（Ｐ＜０．０５），提示：受自由基攻击的细胞膜，其流动性比正常减低，且蛋白质构象发生了改变。加ＥＧｂ保护后，可减少自由基引起的膜流动性及蛋白质构象改变，起到保护神经细胞的作用。经·ＯＨ攻击的神经细胞，其ＬＤＨ活性及死细胞比率都比对照组高，而ＥＧｂ对此有保护作用。本文结果说明一定浓度ＥＧｂ可保护大脑皮层神经细胞免受自由基的损伤。
From the cellular and molecular levels, neuronal cell isolation, spin labeling, enzymatic methods and cell staining techniques were used to investigate the effects of free radicals on nerve cell injury and EGb protection. The results showed that the order parameter S, spin-related time τc, and S / W of the membrane-bound sulfhydryl-labeled membrane were significantly higher (P <0.05) in free radical challenged cells, suggesting that The cell membrane, its mobility lower than normal, and the protein conformation has changed. Plus EGb protection, can reduce free radical-induced membrane fluidity and protein conformational changes, play a role in the protection of nerve cells. Neuronal cells challenged by · OH had higher LDH activity and dead cells ratio than the control group, while EGb had a protective effect. Our results demonstrate that certain concentrations of EGb protect neurons of the cerebral cortex from free radical damage.