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目的 探讨肠道屏障功能损害时应用重组人生长激素(rhGH )对肠道免疫屏障中肠黏膜及固有层内T淋巴细胞亚群和浆细胞的影响。方法 将60只Wistar大鼠雌雄各半随机分为实验组和对照组,按实验天数(2、4、6d)又各分为3组,每组10只,以肠缺血再灌注模型造成肠道屏障功能损害的病理现象,实验组给与rhGH (每日1.3 3U/kg体重) ,观察回肠末端黏膜固有层内CD8+、CD4+、CD3 +T淋巴细胞数及IgA浆细胞的数量变化。结果 (1)实验组CD8+、CD4+、CD3 +T及IgA浆细胞第6天与第2、4天比较数量显著增多,差异有统计学意义(P <0 .0 5 )。(2 )实验组与对照组第6天,CD8+、CD4+T及IgA浆细胞的数量实验组均高于对照组,差异有统计学意义(P <0 .0 5 )。(3 )CD4+/CD8+比值第2天两组差异有统计学意义(P <0 .0 5 )。结论 肠道屏障功能损害时应用重组人生长激素能够调节肠黏膜的免疫屏障,其作用时间在第6天可能最明显
Objective To investigate the effect of recombinant human growth hormone (rhGH) on T lymphocyte subsets and plasma cells in the intestinal mucosa and lamina propria of the intestinal tract after impaired intestinal barrier function. Methods Sixty male and female Wistar rats were randomly divided into experimental group and control group. Each experimental group was divided into 3 groups (2, 4 and 6 days) with 10 rats in each group. The intestine was subjected to intestinal ischemia-reperfusion The experimental group was given rhGH (1.3 3U / kg body weight daily), and the numbers of CD8 +, CD4 +, CD3 + T lymphocytes and IgA plasma cells in the lamina propria of the terminal ileum were observed. Results (1) The numbers of CD8 +, CD4 +, CD3 + T and IgA plasma cells in experimental group increased significantly on the 6th day compared with those on the 2nd and the 4th day, the difference was statistically significant (P <0.05). (2) The number of plasma cells of CD8 +, CD4 + T and IgA in the experimental group and the control group on the 6th day were all higher than that of the control group (P <0.05). (3) The difference of CD4 + / CD8 + ratio between the two groups on the second day was statistically significant (P <0.05). Conclusion The application of recombinant human growth hormone in the functional impairment of the intestinal barrier can regulate the immune barrier of intestinal mucosa, and its action time may be the most obvious on the 6th day