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目的:建立银青口溃含片薄层色谱鉴别和含量测定方法。方法:采用TLC法对方中青黛、冰片两味药进行定性鉴别;采用HPLC法对方中金银花的有效成分绿原酸进行含量测定。结果:本品定性鉴别薄层色谱特征明显,专属性强;方法学考察表明,绿原酸进样量在0.043 2～0.432 0μg范围内具有良好的线性关系(r=0.999 9),平均回收率为100.2%(RSD=1.28%,n=6)。结论:该法简便、准确、重复性好,可用于银青口溃含片的质量控制。
OBJECTIVE: To establish a method for the identification and content determination of Yinqingkou ulcer tablet by TLC. Methods: The contents of chlorogenic acid in Lonicera japonica Thunb. Were determined by TLC. Results: The TLC identification of this product was obvious and specific. The methodological study showed that there was a good linearity (r = 0.999 9) for the injection volume of chlorogenic acid in the range of 0.043 2-0.432 0 μg, the average recovery Was 100.2% (RSD = 1.28%, n = 6). Conclusion: The method is simple, accurate and reproducible, and it can be used for the quality control of Yin Qing Kou ulcer tablets.