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目的 研究脑血栓和 (或 )脑腔隙性梗死 (脑腔梗 )患者中凝血因子Ⅻ (FⅫ )对纤溶的影响。方法 用ELISA方法检测脑血栓患者血浆FⅫ∶Ag、FⅫa、βFⅫa水平 ,FⅫ∶C和其他纤溶活性 ;利用MOEA技术筛选是否存在已知常见的FⅫ基因点突变。结果 10 7例脑血栓患者中 ,有 2 2例呈FⅫ∶C下降 ,类似FⅫCRM+表现 ,PLG∶A、α2 AP∶A升高和D 二聚体 (D D)水平降低的同时 ,FⅫa和βFⅫa水平明显降低 ;2 2例FⅫ∶C下降患者均未见已知的FⅫ基因突变。结论 FⅫ∶C下降可能通过降低纤溶酶原的活性 ,在脑血栓形成中发挥重要作用 ;类似FⅫCRM+的FⅫ异常 ,其基因突变与已知基因突变不同 ;FⅫ 334精氨酸、35 3精氨酸所在区域相应的基因点突变可能对FⅫa、βFⅫa水平和纤溶活性下降更重要。所以血栓性疾病筛选检查中有必要考虑FⅫ的检测
Objective To investigate the effect of coagulation factor Ⅻ (FⅫ) on fibrinolysis in patients with cerebral thrombosis and / or cerebral lacunar infarction (cerebral lacunar infarcts). Methods The levels of FⅫ:Ag, FⅫa, βFⅫa, FⅫ:C and other fibrinolytic activities in patients with cerebral thrombosis were detected by ELISA. The use of MOEA technique to screen for the existence of known FⅫ gene point mutations. Results In 107 cases of cerebral thrombosis, FⅫ: C was decreased in 22 cases, similar to FⅫCRM +. The levels of FⅫa and βFⅫa were decreased while PLG:A, α2 AP:A were increased and D dimer (DD) was decreased Significantly lower; 2 cases of F Ⅻ: C decreased in patients with no known F Ⅻ gene mutations. Conclusions The decrease of FⅫ: C may play an important role in cerebral thrombosis by decreasing the activity of plasminogen. F Ⅻ anomaly similar to F Ⅻ CRM + has a mutation different from that of known genes. F Ⅻ 334 arginine, 35 3 arginine Point mutations in the region where the acid is located may be more important for F? A,? F? A levels and fibrinolytic activity. Therefore, screening for thrombotic disease need to consider F 检 detection