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背景:抗氧化效能的营养素对脂质过氧化反应的抑制作用和对心血管疾病预防作用正日益受到人们的关注。目的:探讨具有清除氧自由基、抗氧化作用的车前子对大鼠脂代谢及抗氧化作用的影响。设计:随机对照实验。单位:河北省新药药理毒理实验室。对象:实验于2004-01/12在河北省新药药理毒理研究实验室完成。40只SD大鼠由河北省实验动物中心提供,健康一级,体质量(210±22)g,雌雄兼用。随机分为5组,空白对照组、阳性对照组、低剂量实验组、中剂量实验组和高剂量实验组,每组8只。方法:空白对照组每天以基础饲料喂养,符合AoAc动物营养标准,自由饮水;阳性对照组每天以高脂饲料喂养,自由饮水;车前子低、中、高剂量组在高脂饲料的基础上分别加入2.5g/kg,5g/kg和15g/kg的车前子,自由饮水。每周称重,记进食量,每隔两周采空腹血测定血清总胆固醇,12周后实验结束,麻醉下腹主动脉采血检测血清血脂水平和超氧化物歧化酶、过氧化氢酶的活性。取血后,立即取出心、肝进行处理,检测其心肌组织中超氧化物歧化酶的活性和脂质过氧化物的含量及肝组织中过氧化氢酶和谷光甘肽过氧化物酶的活性。主要观察指标:大鼠血脂水平和超氧化物歧化酶及过氧化氢酶的活性。结果:纳入40只大鼠均进入结果分析。①血清总胆固醇:车前子高剂量组显著低于阳性对照组犤(1.40±0.13,1.83±0.13)mmol/L,(P<0.05)犦。②血清超氧化物歧化酶活性:阳性对照组明显低于空白对照组犤(174.29±10.33,193.19±.7813)NU/mg,(P<0.05)犦。③心肌组织中脂质过氧化物含量:阳性对照组显著高于空白对照组犤(3.64±0.26,2.91±0.50)mmol/mg,(P<0.05)犦;车前子中剂量组显著低于阳性对照组犤(3.13±0.26,3.64±0.26)mmoL/mg,(P<0.05)犦。④肝组织中过氧化氢酶活性:阳性对照组明显低于正常对照组犤(34.64±3.26,44.72±2.67)NU/mg,(P<0.05)犦;车前子中剂量组明显高于阳性对照组犤(44.84±3.79,34.96±3.64)Nu/mg,(P<0.05)犦。结论:车前子可降低大鼠血清总胆固醇、三酰甘油和脂质过氧化物水平并提高超氧化物歧化酶活性,在浓度为15g/kg时车前子清除氧自由基、抗氧化的作用最明显,可减轻脂质代谢紊乱。
BACKGROUND: The inhibition of lipid peroxidation by nutrients with antioxidant activity and the prevention of cardiovascular disease are increasingly attracting attention. Objective: To investigate the effects of psyllium with scavenging oxygen free radicals and anti-oxidation on lipid metabolism and antioxidation in rats. Design: Randomized controlled trials. Unit: Hebei Province New Drug Pharmacology and Toxicology Laboratory. PARTICIPANTS: The experiment was performed at the New Drug Pharmacology and Toxicology Research Laboratory of Hebei Province from January to December 2004. Forty SD rats were provided by the Experimental Animal Center of Hebei Province. They were of a healthy grade and had a body weight of (210±22) g. Randomly divided into 5 groups, blank control group, positive control group, low-dose experimental group, middle-dose experimental group and high-dose experimental group, 8 in each group. Methods: The blank control group was fed with basal diet every day, which accorded with AoAc animal nutrition standard and free drinking water. The positive control group was fed with high-fat diet every day, and was free to drink water; the low-, medium-, and high-dose group of psyllium was based on high-fat diet. Separately add 2.5g/kg, 5g/kg and 15g/kg of plantago to freely drink water. Weighed weekly, recorded food intake, fasting blood every two weeks to determine serum total cholesterol, 12 weeks after the end of the experiment, abdominal anesthesia blood anesthesia to detect serum lipid levels and superoxide dismutase, catalase activity. After blood was taken, the heart and liver were removed and processed to determine the superoxide dismutase activity and lipid peroxide content in the myocardial tissue and the activity of catalase and glutathione peroxidase in the liver tissue. MAIN OUTCOME MEASURES: Serum lipid levels and activities of superoxide dismutase and catalase in rats. RESULTS: All 40 rats were included in the result analysis. 1 serum total cholesterol: psyllium high dose group was significantly lower than the positive control group 犤 (1.40 ± 0.13, 1.83 ± 0.13) mmol / L, (P <0.05) 犦. 2 Serum superoxide dismutase activity: The positive control group was significantly lower than the blank control group (174.29±10.33, 193.19±.7813) NU/mg, (P<0.05). 3 Lipid peroxide content in myocardial tissue: The positive control group was significantly higher than the blank control group (3.64±0.26, 2.91±0.50) mmol/mg, (P<0.05); the middle vehicle dose group was significantly lower Positive control group 犤(3.13±0.26, 3.64±0.26) mmoL/mg, (P<0.05) 犦. 4The activity of catalase in liver: The positive control group was significantly lower than the normal control group (34.64±3.26, 44.72±2.67) NU/mg, (P<0.05). The psyllium middle dose group was significantly higher than the positive group. Control group 犤(44.84±3.79, 34.96±3.64) Nu/mg, (P<0.05) 犦. Conclusion: Psyllium can reduce serum total cholesterol, triacylglycerol and lipid peroxide levels and increase the activity of superoxide dismutase in rats. At a concentration of 15g/kg, psyllium scavenging oxygen free radicals and antioxidants. The most obvious effect can reduce lipid metabolism disorders.