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Fermentation technology ofCandida BH002. a 1 0-hydroxy-2-decenoic acid producing microorganism, was primarily studied in this paper. Results showed that the optimum medium for the batch-fed fermentation was: sucrose 4%. ammonium sulfate 0.3%. peptone 0. 1%. beef extract 0.1%. dipotassium hydrogen phosphate 0.2%. royal jelly 0.5%. magnesium sulfate 0.05%. ferrous sulfate 0.00 1% and CoCl2 0.00 1% and pH 7.0. The optimum initial pH value, dissoluble oxygen and reducing sugar concentration were 7.0, 25ml/250ml and 7.0% respectively. Meanwhile. the effects of carbon source and metal ions on the 10-hydroxy-2-decenoic acid biosynthesis were also studied primarily.