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1目的 探讨急性白血病 (AL)多药耐药相关蛋白 (MRP)基因的表达及临床意义。 2方法 采用半定量逆转录聚合酶链反应 (RT- PCR)方法 ,对 77例 AL 病人及 10例健康人基因表达进行检测。 3结果 77例 AL病人 MRP平均表达水平与对照组比较差异无显著性 ;复发组 MRP平均表达水平与初治组比较差异有显著性 (t=5 .32 ,P<0 .0 1)。 4结论 AL 的复发与 MRP表达有关 ,MRP基因的过度表达可以作为判断 AL 病人预后的重要指标。
1 Objective To investigate the expression of multidrug resistance-associated protein (MRP) gene in acute leukemia (AL) and its clinical significance. 2 Methods Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the gene expression in 77 AL patients and 10 healthy controls. 3 Results The average expression level of MRP in 77 patients with AL was not significantly different from that in the control group. The average MRP expression level in the relapse group was significantly lower than that in the initial treatment group (t = 5.32, P <0.01). 4 Conclusion AL recurrence and MRP expression, MRP gene overexpression can be used as an important indicator of prognosis of AL patients.