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目的探讨化学发光免疫分析(CLIA)法联合梅毒螺旋体明胶颗粒凝集试验(TPPA),在临床梅毒血清学筛查中的应用价值。方法用CLIA进行特异性梅毒螺旋体抗体初筛试验,阳性标本再经TPPA和梅毒甲苯胺红不加热血清试验(TRUST)复检。CLIA与TPPA结果不一致者用蛋白印迹法(WB)验证。结果 599例CLIA初筛阳性标本用TPPA法复检阳性568例、阴性31例;TRUST法复检阳性189例、阴性410例。31例TPPA阴性标本经WB试验验证结果为18例阴性、13例阳性。结论 CLIA法联合TPPA法初筛梅毒作为梅毒筛查的流程是可行的,值得推广。
Objective To investigate the value of chemiluminescence immunoassay (CLIA) in combination with Treponema pallidum gelatin particle agglutination test (TPPA) in clinical serological screening of syphilis. Methods Preliminary screening test of specific Treponema pallidum antibody using CLIA was carried out. The positive samples were retested with TRUST by TPPA and syphilis toluidine red unheated serum test. Inconsistent results between CLIA and TPPA were verified by Western blotting (WB). Results 599 cases of CLIA positive screening positive specimens with TPPA retest positive 568 cases, negative 31 cases; TRUST retest positive 189 cases, negative 410 cases. 31 cases of TPPA-negative specimens by the WB test results showed that 18 cases were negative, 13 cases were positive. Conclusion CLIA method combined TPPA screening of syphilis as syphilis screening process is feasible and worth promoting.