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目的探讨Toll样受体-4(TLR4)在内毒素诱导的葡萄膜炎中的表达和意义。设计实验性研究。研究对象Wistar大鼠12只,随机分为模型组(n=6)和对照组(n=6)。方法模型组通过足底及腹腔注射霍乱弧菌内毒素诱导出葡萄膜炎,对照组注射磷酸盐缓冲液。注射后4h、10h、16h、24h裂隙灯观察眼前节反应。注射后24h通过免疫组化方法检测眼球冰冻切片及葡萄膜铺片中TLR4的表达。TLR4阳性判定标准:呈棕黄色颗粒定位于胞膜、胞浆。主要指标眼前节炎症反应程度、葡萄膜铺片中TLR4阳性表达的细胞数量。结果内毒素注射后4h虹膜血管扩张充血,16h前房可见纤维素渗出。模型组虹膜铺片内可见大量TLR4阳性表达细胞,位于虹膜基质层,对照组虹膜铺片内只见少量TLR4阳性表达细胞,模型组虹膜中阳性细胞数量高于对照组(P<0.05)。冰冻切片中虹膜内偶见少量TLR4表达。脉络膜和视网膜冰冻切片及铺片中均未见阳性细胞。结论内毒素诱导的葡萄膜炎中TLR4表达增高,提示TLR4可能在急性前葡萄膜炎的发生发展中具有一定作用。(眼科,2008,17:48-51)
Objective To investigate the expression and significance of Toll-like receptor 4 (TLR4) in endotoxin-induced uveitis. Design experimental research. Twelve Wistar rats were randomly divided into model group (n = 6) and control group (n = 6). Methods In the model group, uveitis was induced by injecting V. cholera endotoxin into the plantar and peritoneal cavity, and phosphate buffered saline was injected into the control group. Four hours after injection, 10h, 16h, 24h slit lamp observed anterior segment reaction. Immunohistochemistry was used to detect the expression of TLR4 in frozen section of eyeball and uveal patch 24 hours after injection. TLR4 positive criteria: brown granules located in the cell membrane, cytoplasm. The main indicators of anterior segment of the degree of inflammatory response, the number of cells in the uveal TLR4 positive expression. Results 4h after endotoxin injection iris vasodilation congestion, 16h anterior chamber visible cellulose exudation. A large number of TLR4 positive cells were found in the iris of the model group, located in the iris layer, only a small number of TLR4 positive cells were observed in the iris of the control group. The number of positive cells in the iris of the model group was higher than that of the control group (P <0.05). A small amount of TLR4 expression was occasionally seen in the iris in frozen sections. There were no positive cells in the choroid and retinal frozen sections and in the patch. Conclusions The increase of TLR4 expression in endotoxin-induced uveitis suggests that TLR4 may play a role in the development of acute anterior uveitis. (Ophthalmology, 2008, 17: 48-51)