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目的:探讨氯化锂对Fmr1基因敲除小鼠(KO鼠)痛觉敏感性的影响及机制。方法:8周龄KO鼠及野生型(WT鼠)小鼠第1天测定热刺激缩足反射潜伏期(paw withdraw thermal latency,PWTL)值作为基础痛阈,第2～5天腹腔注射氯化锂后测定PWTL值,第6天给药后取脊髓腰骶膨大段组织,通过免疫组化及Western blot检测实验小鼠脊髓GSK3β和P-GSK3β水平的变化。结果:KO鼠的PWTL值比WT鼠明显升高。使用氯化锂后,KO鼠PWTL值恢复至WT鼠水平。KO鼠脊髓P-GSK3β表达较WT鼠明显减少。使用氯化锂后,KO鼠脊髓P-GSK3β表达增多。结论:KO鼠热痛觉敏感性降低。KO鼠脊髓P-GSK3β表达减少可能是KO鼠热痛觉敏感性降低的原因之一;氯化锂可能通过增加脊髓中P-GSK3β的表达而改善KO鼠热痛觉敏感性。
Objective: To investigate the effect and mechanism of lithium chloride on the pain sensitivity in Fmr1 knockout mice (KO mice). Methods: Wolff-Parkinson's disease (PWTL) was measured on the first day of 8-week-old KO mice and wild type (WT) mice as the basis of pain threshold. Intraperitoneal injection of lithium chloride After 6 days, the tissues of lumbosacral swollen section of spinal cord were taken out. The changes of GSK3β and P-GSK3β in the spinal cord were detected by immunohistochemistry and Western blot. Results: The PWTL of KO mice was significantly higher than that of WT mice. After using lithium chloride, KO mouse PWTL values restored to WT mouse levels. The expression of P-GSK3β in KO mice spinal cord decreased significantly compared with WT mice. The use of lithium chloride, KO rat spinal cord P-GSK3β expression increased. Conclusion: The pain sensitivity of KO rats decreased. Decreased expression of P-GSK3β in KO rats spinal cord may be one of the reasons for the decrease of thermal hyperalgesia sensitivity in KO rats. Lithium chloride may improve the thermal pain sensitivity of KO rats by increasing the expression of P-GSK3β in the spinal cord.