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目的探讨c-fos诱导生长因子(Figf)对小鼠胰腺再生的影响。方法建立小鼠胰腺损伤模型,于术前、术后12h、24h检测血糖,48h后收集剩余胰腺组织。经全基因组芯片分析、Q-PCR验证Figf发生高表达。构建Figf过表达质粒,转染胰岛内皮细胞(MS 1),36h后ELISA检测上清培养液胰岛素分泌量的变化,Q-PCR检测转染后MS 1中Pdx1、Insulin1mRNA表达水平。结果小鼠胰腺再生过程Figf mRNA表达上调。与未转染组、转染PcDNA 3.1组比较,转染Figf过表达质粒组胰岛素分泌量明显升高[未转染组(116.89±6.09)(P<0.01);转染PcDNA 3.1组(114.24±4.60)(P<0.01)],转染Figf过表达质粒组Pdx1、Insulin1mRNA表达水平均明显升高(P<0.01)。结论 MS 1中过表达Figf可使Pdx1、Insulin1mRNA表达水平升高,增加胰岛素的分泌。Figf可能在胰岛β细胞再生中发挥着重要作用,是小鼠胰腺再生的关键基因。
Objective To investigate the effect of c-fos-induced growth factor (FGF) on the pancreatic regeneration in mice. Methods The mouse model of pancreatic injury was established. Blood glucose was measured at preoperative and postoperative 12h and 24h, and the remaining pancreatic tissue was collected 48h later. The whole genome analysis of the chip, Q-PCR validation Figf high expression. Figf overexpression plasmids were constructed and transplanted into islet endothelial cells (MS 1). After 36 h, the changes of insulin secretion in supernatant medium were detected by ELISA. The expression of Pdx1 and Insulin1 mRNA in MS 1 was detected by Q-PCR. Results Figf mRNA expression in mouse pancreatic regeneration was up-regulated. Compared with untransfected group and pcDNA3.1 transfected group, the insulin secretion of Figf overexpression plasmid group was significantly increased (116.89 ± 6.09, P <0.01) 4.60) (P <0.01). The expression levels of Pdx1 and Insulin1 mRNA in the transfected Figf overexpression plasmid group were significantly increased (P <0.01). Conclusion Overexpression of Figf in MS 1 can increase the expression of Pdx1 and Insulin1 mRNA and increase insulin secretion. Figf may play an important role in pancreatic β-cell regeneration and is a key gene in mouse pancreatic regeneration.