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选用大鼠四血管夹闭的前脑缺血再灌注损伤模型,在损伤后第3~10天腹腔注射给予缬沙坦3 mg/(kg.d)或生理盐水(对照),使用组织学、免疫组织化学和行为学方法检测指标变化。发现给予缬沙坦对大鼠脑内海马CA1短期(损伤后第14天)的神经元存活力和Morris水迷宫行为无显著影响,但是远期(损伤后第56天)能显著增加神经元存活力,且大鼠Morris水迷宫行为表现得到显著改善,同时,CA1区的MAP1B阳性神经突起的数目显著增加。提示缬沙坦有助于改善脑缺血再灌注损伤后大鼠的远期空间认知能力,可能机制是增强了损伤灶的神经元远期存活能力和突起生长能力。
The model of forebrain ischemia-reperfusion injury occluded by four vessels in rats was selected. Valsartan 3 mg / (kg · d) or saline (control) was injected intraperitoneally 3 to 10 days after injury, Immunohistochemical and behavioral methods to detect changes in indicators. Valsartan was found to have no significant effect on neuronal viability and Morris water maze behavior in the hippocampal CA1 short-term (day 14 after injury) in rat brains, but long-term (day 56 post-injury) neuronal memory Vitality, and Morris water maze behavior was significantly improved, while, CA1 area MAP1B positive neurites increased significantly. These results suggest that valsartan can ameliorate the long-term spatial cognitive ability of rats after cerebral ischemia-reperfusion injury. The possible mechanism is that the long-term viability of neurons and the ability of neurite outgrowth in damaged lesions are enhanced.